日本家畜中弓形杆菌属的分布情况。

Distribution of Arcobacter species among livestock in Japan.

作者信息

Kabeya Hidenori, Maruyama Soichi, Morita Yukio, Kubo Masatoshi, Yamamoto Kazuaki, Arai Sachiko, Izumi Taiki, Kobayashi Yoko, Katsube Yasuji, Mikami Takeshi

机构信息

Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-8510, Kanagawa, Japan.

出版信息

Vet Microbiol. 2003 May 19;93(2):153-8. doi: 10.1016/s0378-1135(02)00312-7.

DOI:10.1016/s0378-1135(02)00312-7

PMID:12637003

Abstract

A survey was conducted to examine the distribution of Arcobacter species among livestock in Japan. During May 1999 and May 2000, fecal samples from cattle (n=332) and swine (n=250), chicken cloacal swabs (n=234), and vaginal swabs of cattle (n=61) and swine (n=15) were submitted for the isolation of Arcobacter species. Arcobacter species were isolated from 3.6 and 10.0% of the cattle and swine fecal samples, respectively, along with 14.5% of chicken cloacal swabs. No significant seasonal differences were observed. Species-specific polymerase chain reaction assay showed that A. butzleri was the most prevalent species (83.3, 60.0 and 47.1% of the cattle, swine and chicken isolates, respectively), followed by A. cryaerophilus 1B (16.7, 36.0 and 55.9% of the cattle, swine and chicken isolates, respectively). Of the samples from vaginal swabs, 8.1 and 13.3% were positive for Arcobacter in cattle and swine, respectively. This is the first report demonstrating the distribution of Arcobacter species among livestock in Japan.

摘要

为研究日本家畜中弓形杆菌属的分布情况，开展了一项调查。在1999年5月至2000年5月期间，提交了来自牛（n = 332）和猪（n = 250）的粪便样本、鸡泄殖腔拭子（n = 234）以及牛（n = 61）和猪（n = 15）的阴道拭子，用于分离弓形杆菌属。分别从3.6%的牛粪便样本、10.0%的猪粪便样本以及14.5%的鸡泄殖腔拭子中分离出了弓形杆菌属。未观察到显著的季节性差异。种特异性聚合酶链反应分析表明，布氏弓形杆菌是最常见的菌种（分别占牛、猪和鸡分离株的83.3%、60.0%和47.1%），其次是嗜低温弓形杆菌1B（分别占牛、猪和鸡分离株的16.7%、36.0%和55.9%）。在阴道拭子样本中，牛和猪的弓形杆菌阳性率分别为8.1%和13.3%。这是首份证明日本家畜中弓形杆菌属分布情况的报告。