Wesley I V, Baetz A L
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, Iowa 50010, USA.
Poult Sci. 1999 Apr;78(4):536-45. doi: 10.1093/ps/78.4.536.
Arcobacter butzleri causes human enteritis and is frequently recovered from poultry carcasses. The purpose of this study was to determine 1) the natural distribution of A. butzleri in poultry and 2) its relative pathogenicity in experimentally infected poultry. Cloacal samples (n = 407) were collected on four occasions from three flocks of chickens. Overall, Arcobacter spp. were recovered from 15% of the birds; A. butzleri was identified in 1% of cloacal samples. Three experimental trials were conducted to determine the susceptibility of birds. In Trial 1, 3-d-old chicks (n = 62) were divided into three groups and infected per os with 1) A. butzleri American Type Culture Collection (ATCC) 49616, 2) a suspension of four field strains of A. butzleri isolated from retail purchased chicken, and 3) Campylobacter jejuni (positive control). Arcobacter was not detected in cloacal swabs or in cecal samples of chicks through Day 5 postinfection; C. jejuni was detected in cloacal swabs of all positive control birds. In Trial 2, 5-d-old outbred turkey poults (n = 88) were infected as described above with the addition of a group infected with a suspension of four field strains of A. butzleri from turkey meat. Arcobacter butzleri was recovered from either cloacal swabs or cecal contents of only 6.0% of birds (4 of 67); C. jejuni was recovered from 100% of the positive control birds (n = 21). In Trial 3, 3-d-old turkey poults of the highly inbred Beltsville White strain (n = 141) were experimentally inoculated. In contrast to earlier trials, A. butzleri was recovered overall from the cloacal swabs or tissues of 65% of the turkeys.
布氏弓形杆菌可引发人类肠炎，且经常在家禽屠体中检测到。本研究的目的是确定：1）布氏弓形杆菌在家禽中的自然分布情况；2）其在实验感染家禽中的相对致病性。从三个鸡群中分四次采集泄殖腔样本（n = 407）。总体而言，15%的禽类样本中检测到弓形杆菌属；1%的泄殖腔样本中鉴定出布氏弓形杆菌。进行了三项实验来确定禽类的易感性。在实验1中，将3日龄雏鸡（n = 62）分为三组，分别经口感染：1）布氏弓形杆菌美国典型培养物保藏中心（ATCC）49616；2）从零售购买的鸡肉中分离出的四株布氏弓形杆菌田间菌株的悬液；3）空肠弯曲菌（阳性对照）。在感染后第5天，雏鸡的泄殖腔拭子或盲肠样本中均未检测到弓形杆菌；所有阳性对照禽类的泄殖腔拭子中均检测到空肠弯曲菌。在实验2中，5日龄远交系火鸡雏（n = 88）按上述方法感染，另外增加一组感染从火鸡肉中分离出的四株布氏弓形杆菌田间菌株的悬液。仅6.0%的禽类（67只中的4只）的泄殖腔拭子或盲肠内容物中检测到布氏弓形杆菌；100%的阳性对照禽类（n = 21）中检测到空肠弯曲菌。在实验3中，对高度近交的贝尔茨维尔白火鸡品系的3日龄火鸡雏（n = 141）进行实验接种。与早期实验不同的是，总体上65%的火鸡的泄殖腔拭子或组织中检测到了布氏弓形杆菌。
