Lewus C B, Kaiser A, Montville T J
Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers--the State University, New Brunswick 08903-0231.
Appl Environ Microbiol. 1991 Jun;57(6):1683-8. doi: 10.1128/aem.57.6.1683-1688.1991.
Ten strains of bacteriocin-producing lactic acid bacteria were isolated from retail cuts of meat. These 10 strains along with 11 other bacteriocin-producing lactic acid bacteria were tested for inhibitory activity against psychotrophic pathogens, including four strains of Listeria monocytogenes, two strains of Aeromonas hydrophila, and two strains of Staphylococcus aureus. Inhibition due to acid, hydrogen peroxide, and lytic bacteriophage were excluded. The proteinaceous nature of the inhibitory substance was confirmed by demonstration of its sensitivity to proteolytic enzymes. Eight of the meat isolates had inhibitory activity against all four L. monocytogenes strains. Bacteriocin activity against L. monocytogenes was found in all of the strains obtained from other sources. Activity against A. hydrophila and S. aureus was also common.
从零售肉类的分割部位分离出10株产细菌素的乳酸菌。将这10株菌与另外11株产细菌素的乳酸菌一起，检测它们对嗜冷病原菌的抑制活性，这些病原菌包括4株单核细胞增生李斯特菌、2株嗜水气单胞菌和2株金黄色葡萄球菌。排除了由酸、过氧化氢和裂解性噬菌体引起的抑制作用。通过证明抑制物质对蛋白水解酶敏感，证实了其蛋白质性质。8株从肉类中分离出的菌株对所有4株单核细胞增生李斯特菌均有抑制活性。在从其他来源获得的所有菌株中均发现了针对单核细胞增生李斯特菌的细菌素活性。对嗜水气单胞菌和金黄色葡萄球菌的活性也很常见。
