Expert-Bezançon Nicole, Rabilloud Thierry, Vuillard Laurent, Goldberg Michel E
Unité de Repliement et Modélisation des Protéines, Institut Pasteur, Paris, France.
Biophys Chem. 2003;100(1-3):469-79. doi: 10.1016/s0301-4622(02)00299-5.
Some non-detergent sulfobetaines had been shown to prevent aggregation and improve the yield of active proteins when added to the buffer during in vitro protein renaturation. With the aim of designing more efficient folding helpers, a series of non-detergent sulfobetaines have been synthesized and their efficiency in improving the renaturation of a variety of proteins (E. coli tryptophan synthase and beta-D-galactosidase, hen lysozyme, bovine serum albumin, a monoclonal antibody) have been investigated. Attempts to correlate the structure of each sulfobetaines with its effect on folding revealed some molecular features that appear important in helping renaturation. This enabled us to design and synthesize new non-detergent sulfobetaines that act as potent folding helpers.
一些非离子型磺基甜菜碱已被证明，在体外蛋白质复性过程中添加到缓冲液中时，可防止聚集并提高活性蛋白的产量。为了设计更有效的折叠助剂，已合成了一系列非离子型磺基甜菜碱，并研究了它们在促进多种蛋白质（大肠杆菌色氨酸合成酶和β-D-半乳糖苷酶、鸡溶菌酶、牛血清白蛋白、一种单克隆抗体）复性方面的效率。试图将每种磺基甜菜碱的结构与其对折叠的影响相关联，揭示了一些在帮助复性方面似乎很重要的分子特征。这使我们能够设计和合成作为有效折叠助剂的新型非离子型磺基甜菜碱。
