Venkateswaran Kasthuri, Kempf Michael, Chen Fei, Satomi Masataka, Nicholson Wayne, Kern Roger
Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA.
National Research Institute of Fisheries Science, Food Processing Division, Kanazawa-ku, Yokohama-City, Kanagawa 236-8648, Japan.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):165-172. doi: 10.1099/ijs.0.02311-0.
One of the spore-formers isolated from a spacecraft-assembly facility, belonging to the genus Bacillus, is described on the basis of phenotypic characterization, 16S rDNA sequence analysis and DNA-DNA hybridization studies. It is a Gram-positive, facultatively anaerobic, rod-shaped eubacterium that produces endospores. The spores of this novel bacterial species exhibited resistance to UV, gamma-radiation, H2O2 and desiccation. The 18S rDNA sequence analysis revealed a clear affiliation between this strain and members of the low G+C Firmicutes. High 16S rDNA sequence similarity values were found with members of the genus Bacillus and this was supported by fatty acid profiles. The 16S rDNA sequence similarity between strain FO-92T and Bacillus benzoevorans DSM 5391T was very high. However, molecular characterizations employing small-subunit 16S rDNA sequences were at the limits of resolution for the differentiation of species in this genus, but DNA-DNA hybridization data support the proposal of FO-92T as Bacillus nealsonii sp. nov. (type strain is FO-92T =ATCC BAAM-519T =DSM 15077T).
基于表型特征、16S rDNA序列分析和DNA-DNA杂交研究，对从航天器组装设施中分离出的一种芽孢形成菌（属于芽孢杆菌属）进行了描述。它是一种革兰氏阳性、兼性厌氧、杆状的真细菌，可产生内生孢子。这种新细菌物种的孢子对紫外线、γ辐射、H2O2和干燥具有抗性。18S rDNA序列分析表明该菌株与低G+C革兰氏阳性菌成员之间存在明确的亲缘关系。发现该菌株与芽孢杆菌属成员具有较高的16S rDNA序列相似性值，脂肪酸谱也支持这一点。菌株FO-92T与食苯芽孢杆菌DSM 5391T之间的16S rDNA序列相似性非常高。然而，利用小亚基16S rDNA序列进行的分子特征分析在该属物种分化的分辨率极限上，但是DNA-DNA杂交数据支持将FO-92T鉴定为尼尔森芽孢杆菌新种（模式菌株为FO-92T =ATCC BAAM-519T =DSM 15077T）。
