Zoetendal Erwin G, Plugge Caroline M, Akkermans Antoon D L, de Vos Willem M
Wageningen Centre for Food Sciences, PO Box 557, 6700 AN Wageningen, The Netherlands.
Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):211-215. doi: 10.1099/ijs.0.02362-0.
A novel strictly anaerobic, cellobiose-degrading bacterium, strain CelloT, was isolated from a human faecal sample by combining enrichments in liquid and soft-agar basal media. A noteworthy characteristic was its inability to grow on normal agar plates and in roll tubes. The cells were coccus shaped and non-motile, with an extracellular slime layer. Growth of strain CelloT occurred between 20 and 40degrees C, with optimal growth at 37 degrees C. The pH range for growth was 5-7.5 with an optimum at 6.5. In pure culture, strain CelloT could only grow on a variety of sugars. Glucose was converted to acetate, ethanol and H2. The doubling time on glucose was 0.5 h. In a syntrophic co-culture with Methanospirillum hungatei strain JF-1T, strain CelloT converted glucose to acetate and H2. The G+C content was 59.2 mol%. 16S rDNA analysis revealed that the closest relatives of strain CelloT were two uncultured bacteria from anaerobic digesters, both with 94% 16S rDNA sequence similarity. The closest cultured representatives belong to genera of the bacterial division 'Verrucomicrobia'. The name Victivallis vadensis gen. nov., sp. nov. is proposed for strain CelloT (=DSM 14823T =ATCC BAA-548T).
通过在液体和软琼脂基础培养基中富集培养，从一份人类粪便样本中分离出了一种新型严格厌氧菌——纤维二糖降解菌CelloT菌株。一个值得注意的特征是它无法在普通琼脂平板和滚管中生长。细胞呈球菌状，无运动性，有细胞外黏液层。CelloT菌株在20至40摄氏度之间生长，最适生长温度为37摄氏度。生长的pH范围是5至7.5，最适pH为6.5。在纯培养中，CelloT菌株只能在多种糖类上生长。葡萄糖被转化为乙酸盐、乙醇和氢气。在葡萄糖上的倍增时间为0.5小时。与亨氏甲烷螺菌JF-1T菌株进行共培养时，CelloT菌株将葡萄糖转化为乙酸盐和氢气。G+C含量为59.2摩尔%。16S rDNA分析表明，CelloT菌株最接近的亲缘种是来自厌氧消化器的两种未培养细菌，它们的16S rDNA序列相似性均为94%。最接近的已培养代表菌属于细菌分类“疣微菌门”的属。提议将CelloT菌株（=DSM 14823T =ATCC BAA-548T）命名为Victivallis vadensis gen. nov., sp. nov. 。
