Bryson E C, Levin L, Banchs F, Trope M
Department of Endodontics, School of Dentistry, UNC at Chapel Hill, 27599, USA.
Dent Traumatol. 2003 Apr;19(2):90-5. doi: 10.1034/j.1600-9657.2003.00143.x.
Minocycline is available as a locally administered tetracycline derivative antibiotic (Arestin, OraPharma, Inc., Warminster, PA, USA) which demonstrated antibacterial activity and anti-inflammatory action that may slow down resorptive processes after severe traumatic injuries to the dentition. A total of 48 premolar roots of six mongrel dogs were instrumented in an aseptic fashion with rotary nickel-titanium files and obturated with vertically condensed warm gutta percha. All accesses were sealed with glass ionomer, the roots extracted and then allowed to bench dry for 60 min. Group 1 consisted of 15 roots that were coated with minocycline and replanted and nine roots that were replanted without minocycline. Group 2 consisted of 24 roots, all of which were prepared with a uniform cemental defect down to sound dentin along the lateral aspect of the root, with a high-speed bur following the 60-min dry time. Half of the roots (12) were then coated with minocycline and replanted, and the other 12 roots were replanted without minocycline. After 4 months, the dogs were killed and the roots prepared for histological evaluation. About 5- microm thick cross-sections of the root and surrounding tissue taken every 90 microm were evaluated for healing. In addition, residual root mass was also measured in the roots of group 1 to determine the extent of root structure loss for each treatment method. The roots with and without minocycline treatment showed no significant differences between the remaining root mass or the percentage of favorably healed root surfaces. The use of minocycline is not currently recommended for prevention or attenuation of external root resorption following avulsion in a dog trauma model.
米诺环素是一种可局部给药的四环素类衍生物抗生素（商品名Arestin，由美国宾夕法尼亚州沃敏斯特的奥罗制药公司生产），它具有抗菌活性和抗炎作用，可能会减缓牙列严重创伤后的吸收过程。对6只杂种犬的48颗前磨牙牙根进行无菌操作，用旋转镍钛锉进行根管预备，并用垂直加压热牙胶进行充填。所有根管口均用玻璃离子封闭，然后拔除牙根并在实验台上干燥60分钟。第1组由15颗涂有米诺环素后再植入的牙根和9颗未涂米诺环素直接再植入的牙根组成。第2组由24颗牙根组成，在干燥60分钟后，用高速牙钻沿牙根侧面制备一个均匀的牙骨质缺损直至健康牙本质。然后，其中一半牙根（12颗）涂有米诺环素后再植入，另外12颗牙根未涂米诺环素直接再植入。4个月后，处死犬只，对牙根进行组织学评估。每隔90微米取约5微米厚的牙根及周围组织横断面，评估愈合情况。此外，还测量了第1组牙根的剩余牙根质量，以确定每种治疗方法牙根结构丧失的程度。米诺环素处理组和未处理组在剩余牙根质量或愈合良好的牙根表面百分比方面均无显著差异。在犬创伤模型中，目前不推荐使用米诺环素预防或减轻牙脱位后的牙根外吸收。
