Legido-Quigley Cristina, Marlin Nicola D, Melin Virginie, Manz Andreas, Smith Norman W
Centre for Analytical Sciences, Department of Chemistry, Imperial College London, London, UK.
Electrophoresis. 2003 Mar;24(6):917-44. doi: 10.1002/elps.200390136.
Over the last decade, monoliths or continuous beds have emerged as an alternative to traditional packed-bed columns for use in capillary electrochromatography (CEC) and micro-high performance liquid chromatography (micro-HPLC). Monolithic columns can be divided into two categories: silica-based monolithic columns and rigid organic polymer-based monolithic columns resulting from the polymerization of acrylamide, styrene, acrylate or methacrylate monomers. In this paper, the chemistry and most recent applications of these various types of monoliths in both CEC and micro-HPLC are presented.
在过去十年中，整体柱或连续床已成为毛细管电色谱（CEC）和微高效液相色谱（micro-HPLC）中传统填充柱的替代选择。整体柱可分为两类：硅胶基整体柱和由丙烯酰胺、苯乙烯、丙烯酸酯或甲基丙烯酸酯单体聚合而成的刚性有机聚合物基整体柱。本文介绍了这些不同类型整体柱在CEC和micro-HPLC中的化学性质及最新应用。
