[酵母信使核糖核蛋白的特性研究（作者译）]
[Characterization of messenger ribonucleoproteins from yeasts (author's transl)].
作者信息
Holz H, Scholing L
出版信息
Z Naturforsch C Biosci. 1975 Jul-Aug;30(4):516-9.
PMID:126593
Abstract
A method to produce in 15 min stable sphaeroplasts from yeast of the early log-phase is described. This method was used to isolate polysomes, after specific radioactive labelling of mRNA. The mRNP *-particles, isolated from polysomes, have sedimentation-coefficients from 10 to 80S and a density of 1.4 g/cm3, the polysomes a density of 1.58 g/cm3 and the ribosomes a density of 1.6. g/cm3.
摘要
本文描述了一种在15分钟内从对数生长期早期的酵母中制备稳定球状体的方法。该方法用于在对mRNA进行特定放射性标记后分离多核糖体。从多核糖体中分离出的mRNP *颗粒的沉降系数为10至80S，密度为1.4 g/cm³，多核糖体的密度为1.58 g/cm³，核糖体的密度为1.6 g/cm³。
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