Sprinzak Einat, Sattath Shmuel, Margalit Hanah
Department of Molecular Genetics and Biotechnology, Faculty of Medicine, P.O. Box 12272, The Hebrew University of Jerusalem, 91120, Jerusalem, Israel.
J Mol Biol. 2003 Apr 11;327(5):919-23. doi: 10.1016/s0022-2836(03)00239-0.
Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We show that the reliability of high-throughput yeast two-hybrid assays is about 50%, and that the size of the yeast interactome is estimated to be 10,000-16,600 interactions.
蛋白质-蛋白质相互作用的数据为深入了解活细胞的分子网络提供了有价值的见解。然而，其准确性常常受到质疑，这就需要对其可靠性进行严格评估。本文提供的计算方法提供了一种直观的方式，可直接评估实验确定的相互作用蛋白对数据集中真阳性的比例。我们表明，高通量酵母双杂交试验的可靠性约为50%，并且酵母相互作用组的规模估计为10000 - 16600个相互作用。
