Thoresen S. I., Tverdal A., Havre G., Morberg H.
The Norwegian College of Veterinary Medicine, P.b. 8146 Dep., N-0033 Oslo, Norway.
Vet Clin Pathol. 1995;24(4):129-133. doi: 10.1111/j.1939-165x.1995.tb00954.x.
To assess changes in 24 blood constituents in frozen serum and heparinized plasma, blood samples were drawn from 10 clinically normal German Shepherd army dogs. The storage characteristics of nine enzymes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, lipase), and 15 metabolites and minerals (albumin, bile acids, bilirubin, calcium, cholesterol, creatinine, fructosamine, glucose, magnesium, phosphate, potassium, protein, sodium, triglycerides, urea) were studied. Parallel samples of serum and heparinized plasma were stored for 90 and 240 days at two different storage temperatures, -200 degrees C and -700 degrees C. Sixteen of the 24 analytes (ALP, ALT, amylase, AST, CK, GGT, GLDH, LDH, bile acids, calcium, cholesterol, creatinine, fructosamine, magnesium, phosphate, urea) showed statistically significant (p < 0.05) changes during the storage period related to storage time, storage temperature, and sample type. Seven of the analytes (amylase, GGT, GLDH, LDH, bile acids, fructosamine, magnesium) showed changes of possible clinical importance with mean differences from baseline larger than 20% for the enzymes and 10% for the metabolites and minerals during the storage periods.
为评估冷冻血清和肝素化血浆中24种血液成分的变化，从10只临床正常的德国牧羊犬军犬采集血样。研究了9种酶（碱性磷酸酶、谷丙转氨酶、淀粉酶、谷草转氨酶、肌酸激酶、γ-谷氨酰转移酶、谷氨酸脱氢酶、乳酸脱氢酶、脂肪酶）以及15种代谢物和矿物质（白蛋白、胆汁酸、胆红素、钙、胆固醇、肌酐、果糖胺、葡萄糖、镁、磷酸盐、钾、蛋白质、钠、甘油三酯、尿素）的储存特性。血清和肝素化血浆的平行样本在-20℃和-70℃两种不同储存温度下储存90天和240天。24种分析物中的16种（碱性磷酸酶、谷丙转氨酶、淀粉酶、谷草转氨酶、肌酸激酶、γ-谷氨酰转移酶、谷氨酸脱氢酶、乳酸脱氢酶、胆汁酸、钙、胆固醇、肌酐、果糖胺、镁、磷酸盐、尿素）在储存期间显示出与储存时间、储存温度和样本类型相关的统计学显著（p<0.05）变化。7种分析物（淀粉酶、γ-谷氨酰转移酶、谷氨酸脱氢酶、乳酸脱氢酶、胆汁酸、果糖胺、镁）显示出可能具有临床重要性的变化，在储存期间，酶的平均差异高于基线20%，代谢物和矿物质的平均差异高于基线10%。
