Meléndez Daniela M, Marti Sonia, Faucitano Luigi, Haley Derek B, Schwinghamer Timothy D, Schwartzkopf-Genswein Karen S
Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, AB T1J 4B1, Canada.
IRTA Ruminant Production Program, Caldes de Montbui, 08140 Barcelona, Spain.
Animals (Basel). 2021 Mar 25;11(4):926. doi: 10.3390/ani11040926.
Lactate is a product of anaerobic glycolysis, used in animal research as an indicator of muscle fatigue. Therefore, it has been used as an indicator of cattle response to long distance transportation. The aim of this study was to assess the relationship of L-lactate concentrations measured using a Lactate Scout+ analyzer and a traditional lactate assay colorimetric kit. Blood samples were collected by venipuncture from 96 steers (Black or Red Angus × Hereford/Simmental and Black or Red Angus × Charolais; 247 ± 38.2 kg BW) prior to loading (LO1) and after 36 h of transport, and prior to reloading and after an additional 4 h of road transportation, and on d 1, 2, 3, 5, 14, and 28 after transport. The Lactate Scout+ analyzer strip was dipped in blood at the time of sampling, while blood samples were collected into sodium fluoride tubes for use in the colorimetric analysis. Pearson correlations were calculated to assess the strength of the relationship between the experimental methods for the quantification of L-lactate concentrations. The magnitude and direction of the correlation, and the level of statistical significance varied over the observed time points, ranging from = -0.03 ( = 0.75; LO1) to = 0.75 ( < 0.0001; d 3). The correlation for the pooled data was weak but statistically significant ( = 0.33, < 0.0001). Based on the low magnitude of the correlation due to variability across sampling time points in this study, the Lactate Scout+ analyzer is not a suitable alternative to a lab-based assay (considered the gold standard) for measuring L-lactate in transported cattle.
乳酸是无氧糖酵解的产物，在动物研究中用作肌肉疲劳的指标。因此，它被用作牛对长途运输反应的指标。本研究的目的是评估使用Lactate Scout+分析仪和传统乳酸测定比色试剂盒测得的L-乳酸浓度之间的关系。在装载前（LO1）、运输36小时后、重新装载前以及额外4小时公路运输后，以及运输后第1、2、3、5、14和28天，通过静脉穿刺从96头阉牛（黑色或红色安格斯×赫里福德/西门塔尔以及黑色或红色安格斯×夏洛来；体重247±38.2千克）采集血样。采样时将Lactate Scout+分析仪试纸条浸入血液中，同时将血样收集到氟化钠管中用于比色分析。计算Pearson相关性以评估定量L-乳酸浓度的实验方法之间关系的强度。相关性的大小和方向以及统计显著性水平在观察的时间点上有所不同，范围从r = -0.03（P = 0.75；LO1）到r = 0.75（P < 0.0001；第3天）。汇总数据的相关性较弱但具有统计学显著性（r = 0.33，P < 0.0001）。基于本研究中采样时间点之间变异性导致的低相关性，Lactate Scout+分析仪不是用于测量运输牛中L-乳酸的基于实验室检测（被视为金标准）的合适替代方法。
