Baron J M, Merk H F, Heise R
Hautklinik, Universitätsklinikum der RWTH Aachen.
Hautarzt. 2003 Apr;54(4):315-20. doi: 10.1007/s00105-003-0514-5. Epub 2003 Mar 11.
Because of their variable application, microarrays are currently used in different areas of research and development, such as skin pharmacology and allergology. Microarrays are plane carriers, on whose surface a variety of known DNA-molecules and proteins were immobilised. Transcripts can be detected by cDNA- and oligonucleotid-arrays and proteins of activated genes can be discovered using antibody microarrays. Detection of allergen-specific IgE from human serum can be performed using allergen chips. Since many details of the molecular mechanism and pathogenesis of skin cancer and inflammatory skin diseases and the effect of xenobiotics on cells of the human skin are still not known, array-technologies are a powerful tool to identify novel marker genes and offer the possibility of develop new therapeutic strategies as well as prognosis- and diagnosis-systems.
由于其应用的多样性，微阵列目前被用于不同的研发领域，如皮肤药理学和变态反应学。微阵列是平面载体，其表面固定有多种已知的DNA分子和蛋白质。转录本可以通过cDNA和寡核苷酸阵列进行检测，活化基因的蛋白质可以使用抗体微阵列来发现。使用过敏原芯片可以检测人血清中的过敏原特异性IgE。由于皮肤癌和炎症性皮肤病的分子机制和发病机制以及外源性物质对人类皮肤细胞的影响的许多细节仍然未知，阵列技术是识别新的标记基因的有力工具，并为开发新的治疗策略以及预后和诊断系统提供了可能性。
