Shockley Keith R, Ward Donald E, Chhabra Swapnil R, Conners Shannon B, Montero Clemente I, Kelly Robert M
Department of Chemical Engineering, North Carolina State University, Raleigh 27695-7905, USA.
Appl Environ Microbiol. 2003 Apr;69(4):2365-71. doi: 10.1128/AEM.69.4.2365-2371.2003.
Collective transcriptional analysis of heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus was examined by using a targeted cDNA microarray in conjunction with Northern analyses. Differential gene expression suggests that P. furiosus relies on a cooperative strategy of rescue (thermosome [Hsp60], small heat shock protein [Hsp20], and two VAT-related chaperones), proteolysis (proteasome), and stabilization (compatible solute formation) to cope with polypeptide processing during thermal stress.
利用靶向cDNA微阵列结合Northern分析,对嗜热古菌激烈火球菌热休克反应进行了集体转录分析。差异基因表达表明,激烈火球菌依靠救援协同策略(热体[Hsp60]、小热休克蛋白[Hsp20]和两种与VAT相关的伴侣蛋白)、蛋白水解(蛋白酶体)和稳定化(相容性溶质形成)来应对热应激期间的多肽加工。