Daniel M R, Wakerley C L
Br J Exp Pathol. 1976 Apr;57(2):137-47.
As a step towards the definition of a medium suitable for long-term storage of potential organ transplants, a study was made of the effects of various solutions on the viability of monolayers of cells stored at 4 degrees. It was found that, for these to survive for more than a few hours, an essential component of the medium was a non-toxic solute which did not cross the cell membrane at low temperatures and could therefore counterbalance the osmotic effect of the intracellular proteins. A high K+/Na+ ratio and hyperosmoticity, separately or together, were ineffective in the absence ofsuch a solute and unnecessary in its presence. The most effective non-permeating solute tested was polyethylene glycol, mol. wt. 20,000 cells stored in a 1% solution of this in phosphate buffered balanced salt solution remained viable for at least 24 hours and could subsequently be grown in culture.
作为确定适合长期保存潜在器官移植的培养基的第一步，研究了各种溶液对4℃保存的单层细胞活力的影响。结果发现，要使这些细胞存活数小时以上，培养基的一个关键成分是一种无毒溶质，它在低温下不会穿过细胞膜，因此可以抵消细胞内蛋白质的渗透作用。在没有这种溶质的情况下，单独或共同的高K⁺/Na⁺比值和高渗性是无效的，而在有这种溶质的情况下则是不必要的。测试的最有效的非渗透性溶质是聚乙二醇，分子量20000。储存在1%该溶液中的磷酸盐缓冲平衡盐溶液中的细胞至少存活24小时，随后可以在培养中生长。
