Kubo Hideyuki, Iritani Nobuhiro, Seto Yoshiyuki
Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Osaka, Japan.
J Med Virol. 2003 Feb;69(2):273-8. doi: 10.1002/jmv.10278.
Genotypes of 44 wild-type measles virus (MV) strains isolated in Osaka, Japan, during 1997-2001, were determined based on phylogenetic analyses of a 456-nt 3' terminal nucleoprotein gene sequence with the reference MV strains designated by the World Health Organization. The wild-type MV strains were classified into two genotypes, D3 and D5, recognized as indigenous in Japan. Six of 12 strains isolated in 1997 were classified into genotype D3 and the other 6 into D5. Eleven of 13 strains were D3, and 2 were D5 in 1998. There were no measles epidemics, and no strains were isolated in 1999. Nine of 10 strains were genotype D5, and only one was D3 in 2000, and 9 of 9 were D5 in 2001. These results indicate that the wild-type MV strains classified into genotypes D3 and D5 co-circulated without the complete change of the MV genotype in Osaka, except in 2001. Furthermore, the prevailing genotype was different between 1998 and 2000-2001. Together with a previous report about MV genotype in this area during 1993-1995, these results suggest that the mutual change of the prevailing wild-type MV genotypes between D3 and D5 occurs every few years in Osaka, Japan.
基于对456个核苷酸的3'末端核蛋白基因序列进行系统发育分析，并与世界卫生组织指定的参考麻疹病毒（MV）毒株进行比较，确定了1997年至2001年期间在日本大阪分离出的44株野生型麻疹病毒（MV）毒株的基因型。这些野生型MV毒株被分为两种基因型，D3和D5，这两种基因型在日本被认为是本土基因型。1997年分离出的12株毒株中，6株被分类为D3基因型，另外6株为D5基因型。1998年的13株毒株中，11株为D3基因型，2株为D5基因型。1999年没有麻疹疫情，也没有分离出毒株。2000年的10株毒株中，9株为D5基因型，只有1株为D3基因型，2001年的9株毒株均为D5基因型。这些结果表明，除2001年外，在大阪，分类为D3和D5基因型的野生型MV毒株共同流行，MV基因型没有完全改变。此外，1998年和2000 - 2001年期间流行的基因型不同。结合之前关于该地区1993 - 1995年期间MV基因型的报告，这些结果表明，在日本大阪，流行的野生型MV基因型在D3和D5之间每隔几年就会相互变化。
