Shimoi Kayoko, Yoshizumi Kazuma, Kido Taketoshi, Usui Yumiko, Yumoto Takashi
Institute for Environmental Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, Yada 52-1, Shizuoka 422-8526, Japan.
J Agric Food Chem. 2003 Apr 23;51(9):2785-9. doi: 10.1021/jf026108a.
Quercetin, rutin, alphaG-rutin (a water soluble flavonoid), and a mixture of rutin and alphaG-rutin were administered to rats by a single gastric intubation, and their absorption and urinary excretion were examined. The plasma and 24 h urinary levels of aglycons (quercetin and tamarixetin/isorhamnetin) were measured by HPLC after deconjugation with beta-glucuronidase/sulfatase treatment. alphaG-rutin was absorbed more rapidly than quercetin or rutin, and the plasma concentrations of quercetin and tamarixetin/isorhamnetin reached the highest peak level 30 min after dosing. Quercetin, rutin, and the mixture of rutin and alphaG-rutin showed the first peak level 8 h, 8 h, and 30 min after dosing, respectively. The area under the concentration-time curve (AUC) for quercetin in rats administered alphaG-rutin was approximately 4.5- and 2-fold higher than those in rats administered quercetin and rutin, respectively, and was almost the same as that in rats administered a mixture of rutin and alphaG-rutin. The highest 24 h urinary excretion was observed in alphaG-rutin-administered rats. These results suggest that alphaG-rutin is absorbed more efficiently than either quercetin or rutin and that a high plasma concentration can be maintained by supplying rutin and alphaG-rutin in combination.
通过单次胃插管法给大鼠施用槲皮素、芦丁、αG-芦丁（一种水溶性类黄酮）以及芦丁和αG-芦丁的混合物，并检测它们的吸收和尿排泄情况。在用β-葡萄糖醛酸酶/硫酸酯酶处理进行去结合后，通过高效液相色谱法测量苷元（槲皮素和柽柳素/异鼠李素）的血浆和24小时尿液水平。αG-芦丁比槲皮素或芦丁吸收更快，给药后30分钟时，槲皮素和柽柳素/异鼠李素的血浆浓度达到最高峰水平。槲皮素、芦丁以及芦丁和αG-芦丁的混合物分别在给药后8小时、8小时和30分钟时出现第一个峰值水平。给予αG-芦丁的大鼠中槲皮素的浓度-时间曲线下面积（AUC）分别比给予槲皮素和芦丁的大鼠高约4.5倍和2倍，并且与给予芦丁和αG-芦丁混合物的大鼠几乎相同。给予αG-芦丁的大鼠24小时尿排泄量最高。这些结果表明，αG-芦丁比槲皮素或芦丁吸收更有效，并且通过联合供应芦丁和αG-芦丁可以维持较高的血浆浓度。
