Stadtländer C, Kirchhoff H
Institut für Mikrobiologie und Tierseuchen der Tierärztlichen Hochschule Hannover, Federal Republic of Germany.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;269(3):355-65. doi: 10.1016/s0176-6724(88)80179-2.
Mycoplasma (M.) mobile 163 K, isolated from the gills of a tench (Tinca tinca L.), was examined for cytotoxic capacities using tracheal organ cultures from gnotobiotic rats and gnotobiotic piglets in pH-controlled experiments (pH 7.2-7.6). The mycoplasmas caused an inhibition of the ciliary activity at incubation temperatures of 20, 25 and 30 degrees C. The strongest cilia stopping effect was observed at an incubation temperature of 25 degrees C, the optimal growth temperature of the mycoplasmas. No ciliostasis occurred at 37 degrees C. The number of the organisms did clearly affect the severity of ciliostasis in the range from 4.0 x 10(4) to 7.7 x 10(8) c.f.u. in rat as well as in porcine tracheal organ cultures. Toxic substances, secreted by the mycoplasmas into the culture medium, could not be detected. In histological investigations cytopathological changes were observed in the epithelial cells, apparent in the destruction and loss of cilia, cytoplasmatic vacuolization, swelling of mitochondria, peripheral orientation of the nuclear chromatin and detachment of epithelial cells from each other and the basal membrane. The final stage of the infection was characterized by complete exfoliation of the epithelial cells and the complete destruction of the multi-layer epithelium. The localization of the mycoplasmas attached to the ciliary epithelium was shown by scanning and transmission electron microscopy.
从丁鱥（Tinca tinca L.）鳃中分离出的运动支原体（Mycoplasma (M.) mobile）163 K，在pH值控制实验（pH 7.2 - 7.6）中，使用无菌大鼠和无菌仔猪的气管器官培养物检测其细胞毒性能力。支原体在20、25和30摄氏度的孵育温度下会抑制纤毛活动。在25摄氏度（支原体的最佳生长温度）的孵育温度下观察到最强的纤毛停止效应。在37摄氏度时未发生纤毛停滞。在大鼠和猪气管器官培养物中，生物体数量在4.0×10⁴至7.7×10⁸ c.f.u.范围内确实会明显影响纤毛停滞的严重程度。未检测到支原体分泌到培养基中的有毒物质。在组织学研究中，在上皮细胞中观察到细胞病理学变化，表现为纤毛的破坏和丧失、细胞质空泡化、线粒体肿胀、核染色质的周边定位以及上皮细胞彼此之间和与基底膜的分离。感染的最后阶段的特征是上皮细胞完全脱落和多层上皮的完全破坏。通过扫描电子显微镜和透射电子显微镜显示了附着在纤毛上皮上的支原体的定位。
