通过玻璃吸附色谱法纯化葡萄球菌α毒素
Purification of staphylococcal alpha-toxin by adsorption chromatography on glass.
作者信息
Cassidy P, Harshman S
出版信息
Infect Immun. 1976 Mar;13(3):982-6. doi: 10.1128/iai.13.3.982-986.1976.
Abstract
Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2% of other forms.
摘要
使用可控孔径玻璃珠上的吸附色谱法从金黄色葡萄球菌生长培养基中纯化葡萄球菌α毒素。用各种阴离子从未修饰的玻璃珠表面洗脱α毒素，洗脱顺序通常遵循离液序列。通过玻璃珠色谱法纯化的α毒素由单一电泳形式组成，其他形式的含量不到2%。
Zotero 插件