Driskell J A, Geders J M, Urban M C
J Lab Clin Med. 1976 May;87(5):813-21.
Vitamin B6 intakes of 33 males, 73 females, and 46 females using oral contraceptives, all 18- to 25-year-old students, were compared to coenzyme stimulation of erythrocyte alanine aminotransferase activities of blood samples obtained from these subjects in order to ascertain the vitamin B6 status and perhaps the requirements of this population group. Trained interviewers obtained 24-hour recalls; subjects supplied 2-day food records. Dietary record analyses revealed that students reported consuming slightly less kilocalories, more protein, and considerably less vitamin B6 than the 1974 recommended dietary allowances. Erthrocyte alanine aminotransferase activities, with and without additional coenzyme, of blood samples from the 3 groups were significantly different from each other; per cent coenzyme stimulation of enzymatic activity of females and females on oral contraceptives were significantly different from each other. Enzyme data were not significantly affected by race, age, income, food budget, preparation of own food, frequency of eating out, or vitamin supplementation. Several subjects in all 3 groups had high per cent stimulation values which is considered to be indicative of subclinical vitamin B6 deficiency. Subjects with values near 0 per cent stimulation had varying vitamin B6 intakes thus perhaps indicating large individual variations in vitamin B6 requirements.
对33名男性、73名女性以及46名服用口服避孕药的女性(均为18至25岁的学生)的维生素B6摄入量进行了比较,并测定了从这些受试者采集的血样中红细胞丙氨酸转氨酶活性的辅酶刺激情况,以确定该人群的维生素B6状态以及可能的需求量。训练有素的访谈人员获取了24小时膳食回顾信息;受试者提供了两天的饮食记录。膳食记录分析显示,学生报告的卡路里摄入量略低,蛋白质摄入量较高,而维生素B6摄入量则远低于1974年推荐的膳食摄入量。三组受试者血样在添加和未添加辅酶情况下的红细胞丙氨酸转氨酶活性存在显著差异;女性及服用口服避孕药女性的酶活性辅酶刺激百分比也存在显著差异。酶数据不受种族、年龄、收入、食物预算、自制食物情况、外出就餐频率或维生素补充剂的显著影响。所有三组中的几名受试者具有较高的刺激百分比值,这被认为表明存在亚临床维生素B6缺乏。刺激值接近0%的受试者维生素B6摄入量各不相同,因此可能表明维生素B6需求量存在较大个体差异。