Xiao Jiang-Wei, Chen Jia-Hui, Ren Ming-Yang, Tian Xiao-Bing, Wang Chong-Shu
Department of General Surgery, the Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Asian Pac J Cancer Prev. 2012;13(11):5897-901. doi: 10.7314/apjcp.2012.13.11.5897.
The aim of the study was to clarify the role of gastrokine 1 in the process of formation and development of gastric cancer. The expression of gastrokine 1 in gastric cancer and corresponding non-cancerous gastric tissues of 52 gastric cancer patients was assessed with the real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry. We also analyzed the relationship between the expression level and clinicopathological characteristics. Gastrokine 1 gene and protein expression in gastric cancer tissues was in both cases significantly lower than in corresponding non-cancerous gastric tissues (both P<0.01), but no significant relationship was found with clinicopathological parameters including tumor location, depth of invasion, differentiation, lymph node metastasis, stage, gender, age and carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9) level in peripheral blood preoperation of patients (P>0.05, respectively). Furthermore, gastrokine 1 gene expression was markedly lower in gastric cancer tissues of Helicobacter pylori (HP)-positive patients than negative ones (P<0.05). The result of the study showed that gastrokine 1 might play a significant role in the process of formation and development of gastric cancer as an anti-oncogene. Its effect might be weakened by HP infection.
本研究旨在阐明胃动素1在胃癌发生发展过程中的作用。采用实时荧光定量聚合酶链反应（RT-PCR）和免疫组织化学方法，检测52例胃癌患者癌组织及相应癌旁胃组织中胃动素1的表达情况，并分析其表达水平与临床病理特征的关系。结果显示，胃癌组织中胃动素1基因及蛋白表达均显著低于相应癌旁胃组织（均P<0.01），但与肿瘤部位、浸润深度、分化程度、淋巴结转移、分期、性别、年龄及术前外周血癌胚抗原（CEA）、糖类抗原19-9（CA19-9）水平等临床病理参数均无明显相关性（均P>0.05）。此外，幽门螺杆菌（HP）阳性患者胃癌组织中胃动素1基因表达明显低于HP阴性患者（P<0.05）。研究结果表明，胃动素1作为一种抑癌基因，可能在胃癌发生发展过程中发挥重要作用，HP感染可能削弱其作用。
