Romero Carlos, Pedryc Andrzej, Muñoz Verónica, Llácer Gerardo, Badenes Maria Luisa
Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial 46113 Moncada, Valencia, Spain.
Genome. 2003 Apr;46(2):244-52. doi: 10.1139/g02-128.
Forty apricot cultivars with different geographic origins belonging to the germplasm collections of St. Istvan University (Budapest, Hungary) and the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were studied by means of SSR markers. The aim of the study was to determine the genetic relationships among genotypes from different eco-geographical groups. Sixteen primer pairs flanking microsatellite sequences in the peach genome were assayed. Eleven of them were polymorphic in the set of cultivars studied and allowed every genotype to be unambiguously distinguished. Genetic diversity in the population studied was analyzed using several variability parameters. A total of 34 alleles were detected with a mean value of 3.1 alleles/locus. The expected heterozygosity mean was 0.46 and the observed heterozygosity was 32% on an average leading to a high value of the Wright's fixation index (0.32). Additionally, UPGMA cluster analysis based on Nei's genetic distance grouped genotypes according to their geographic origins and pedigrees. SSR markers have proved to be an efficient tool for fingerprinting cultivars and conducting genetic-diversity studies in apricot.
利用SSR标记对来自匈牙利布达佩斯圣伊什特万大学种质资源库和西班牙巴伦西亚农业研究所（IVIA）（巴伦西亚）的40个不同地理来源的杏品种进行了研究。该研究的目的是确定不同生态地理群体中基因型之间的遗传关系。检测了16对位于桃基因组微卫星序列侧翼的引物对。其中11对在研究的品种组中具有多态性，可明确区分每个基因型。使用多个变异性参数分析了所研究群体的遗传多样性。共检测到34个等位基因，平均每个位点3.1个等位基因。预期杂合度均值为0.46，观察到的杂合度平均为32%，导致赖特固定指数值较高（0.32）。此外，基于内氏遗传距离的UPGMA聚类分析根据基因型的地理来源和系谱对其进行了分组。SSR标记已被证明是用于杏品种指纹识别和进行遗传多样性研究的有效工具。
