荚膜红细菌的单一超氧化物歧化酶是一种兼性含锰酶。
The single superoxide dismutase of Rhodobacter capsulatus is a cambialistic, manganese-containing enzyme.
作者信息
Tabares Leandro C, Bittel Cristian, Carrillo Néstor, Bortolotti Ana, Cortez Néstor
机构信息
Instituto de Biología Molecular y Celular de Rosario, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina.
出版信息
J Bacteriol. 2003 May;185(10):3223-7. doi: 10.1128/JB.185.10.3223-3227.2003.
Abstract
The phototrophic bacterium Rhodobacter capsulatus contains a single, oxygen-responsive superoxide dismutase (SOD(Rc)) homologous to iron-containing superoxide dismutase enzymes. Recombinant SOD(Rc), however, displayed higher activity after refolding with Mn(2+), especially when the pH of the assay mixture was raised. SOD(Rc) isolated from Rhodobacter cells also preferentially contains manganese, but metal discrimination depends on the culture conditions, with iron fractions increasing from 7% in aerobic cultures up to 40% in photosynthetic cultures. Therefore, SOD(Rc) behaves as a Mn-containing dismutase with cambialistic properties.
摘要
光合细菌荚膜红细菌含有一种单一的、对氧有反应的超氧化物歧化酶（SOD(Rc)），它与含铁超氧化物歧化酶同源。然而，重组SOD(Rc)在用Mn(2+)重折叠后表现出更高的活性，特别是当测定混合物的pH值升高时。从荚膜红细菌细胞中分离出的SOD(Rc)也优先含有锰，但金属的区分取决于培养条件，铁的比例从需氧培养中的7%增加到光合培养中的40%。因此，SOD(Rc)表现为一种具有兼性特性的含锰歧化酶。
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