Nelson Susan M, Cartwright Charles P
Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, Minneapolis, Minnesota 55415, USA>
J Clin Microbiol. 2003 May;41(5):2141-3. doi: 10.1128/JCM.41.5.2141-2143.2003.
The ability of a fluconazole-containing agar screen assay to accurately detect isolates of Candida glabrata resistant to the azole antifungal agent fluconazole was evaluated on a collection of 100 clinical isolates of this organism. Results were correlated with the MIC of fluconazole for these isolates and compared with the results of a previously published disk diffusion-based fluconazole resistance screening test. Agar screen assay results were in categorical agreement with MIC-based determinations for 97% (97/100) of the isolates tested. This correlation was higher than that obtained with the disk diffusion technique, which categorized only 87% (87/100) of isolates correctly, and suggests that the agar screening approach can effectively expedite fluconazole susceptibility testing of C. glabrata isolates.
在100株光滑念珠菌临床分离株中，评估了含氟康唑的琼脂筛选试验准确检测对唑类抗真菌药氟康唑耐药的光滑念珠菌分离株的能力。将结果与这些分离株的氟康唑MIC进行关联，并与先前发表的基于纸片扩散法的氟康唑耐药性筛选试验结果进行比较。琼脂筛选试验结果与97%（97/100）受试分离株基于MIC的测定结果完全一致。这种相关性高于纸片扩散技术，后者仅能正确分类87%（87/100）的分离株，这表明琼脂筛选方法可以有效地加快光滑念珠菌分离株的氟康唑敏感性检测。
