Kirkpatrick W R, Turner T M, Fothergill A W, McCarthy D I, Redding S W, Rinaldi M G, Patterson T F
Departments of Medicine, The University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284, USA.
J Clin Microbiol. 1998 Nov;36(11):3429-32. doi: 10.1128/JCM.36.11.3429-3432.1998.
We describe a simple procedure for detecting fluconazole-resistant yeasts by a disk diffusion method. Forty clinical Candida sp. isolates were tested on RPMI-glucose agar with either 25- or 50-microgram fluconazole disks. With 25-microgram disks, zones of inhibition of >/=20 mm at 24 h accurately identified 29 of 29 isolates for which MICs were </=8 microgram/ml, and with 50-microgram disks, zones of >/=27 mm identified 28 of 29 such isolates. All 11 isolates for which MICs were >8 microgram/ml were identified by using either disk. Disk diffusion may be a useful screening method for clinical microbiology laboratories.
我们描述了一种通过纸片扩散法检测氟康唑耐药酵母的简单程序。对40株临床念珠菌属分离株在含有25微克或50微克氟康唑纸片的RPMI - 葡萄糖琼脂上进行测试。使用25微克纸片时，24小时抑菌圈直径≥20毫米可准确鉴定出29株中29株最低抑菌浓度（MIC）≤8微克/毫升的分离株；使用50微克纸片时，抑菌圈直径≥27毫米可鉴定出29株中28株此类分离株。所有11株MIC＞8微克/毫升的分离株使用任一纸片均可鉴定出来。纸片扩散法可能是临床微生物实验室一种有用的筛查方法。
