Tohoku Medical Megabank Organization, Tohoku University, Sendai, Japan.
Department of International Environmental and Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Sci Rep. 2021 Apr 23;11(1):8878. doi: 10.1038/s41598-021-87384-8.
The plant root-associated environments such as the rhizosphere, rhizoplane, and endosphere are different from the outer soil region (bulk soil). They establish characteristic conditions including microbiota, metabolites, and minerals, and they can directly affect plant growth and development. However, comprehensive insights into those characteristic environments, especially the rhizosphere, and molecular mechanisms of their formation are not well understood. In the present study, we investigated the spatiotemporal dynamics of the root-associated environment in actual field conditions by multi-omics analyses (mineral, microbiome, and transcriptome) of soybean plants. Mineral and microbiome analyses demonstrated a characteristic rhizosphere environment in which most of the minerals were highly accumulated and bacterial communities were distinct from those in the bulk soil. Mantel's test and co-abundance network analysis revealed that characteristic community structures and dominant bacterial taxa in the rhizosphere significantly interact with mineral contents in the rhizosphere, but not in the bulk soil. Our field multi-omics analysis suggests a rhizosphere-specific close association between the microbiota and mineral environment.
植物根系相关的环境，如根际、根面和根内环境，与外部土壤区域（土壤体）不同。它们建立了包括微生物群、代谢物和矿物质在内的特征条件，并能直接影响植物的生长和发育。然而，人们对这些特征环境，特别是根际，以及它们形成的分子机制的全面了解还很有限。在本研究中，我们通过对大豆植物的多组学分析（矿物质、微生物组和转录组），研究了实际田间条件下根系相关环境的时空动态。矿物质和微生物组分析表明，根际环境具有特征性，其中大部分矿物质高度积累，细菌群落与土壤体中的群落明显不同。Mantel 检验和共同丰度网络分析表明，根际中特征性的群落结构和优势细菌类群与根际中的矿物质含量显著相互作用，但与土壤体中的矿物质含量没有显著相互作用。我们的田间多组学分析表明，微生物群和矿物质环境之间存在根际特有的紧密联系。
