Meyts Isabelle, Proesmans Marijke, Gerven Veerle Van, Hoppenbrouwers Karel, De Boeck Kris
Paediatric Pulmonology Department, University Hospital Gasthuisberg, Herestraat 49, 3000, Leuven, Belgium.
Youth Health Care Centre, Centre for Preventive Medicine, Leuven, Belgium.
Eur J Pediatr. 2003 Jul;162(7-8):506-510. doi: 10.1007/s00431-003-1215-x. Epub 2003 May 9.
Exhaled nitric oxide (ENO) is used as a non-invasive marker of airway inflammation. The aim of this study was to measure ENO in a pre-school population using a relatively novel method, the off-line tidal breathing method, and to investigate differences in ENO between subjects with different presentations of wheezing. ENO was measured in 129 children (median age 4.4 years, quartiles 4.0-4.8 years) through a mouth mask attached to a two-way valve with an expiratory resistance of 5 cm H(2)0. Mean tidal ENO concentration (tENO) was calculated from triplicate measurements. Mean +/- SEM tENO for 89 control subjects was 13+/-0.4 ppb (95%CI 11.8-13.7 ppb); this level was significantly different from tENO in 15 children with a history of recurrent wheezing (18.6+/-1.9 ppb; 95%CI 14.5-22.7 ppb; t-test P<0.0001). Mean tENO in 16 children with a single wheezing episode was 11.4+/-1.0 ppb (95%CI 9.2-13.6 ppb) and thus significantly different from the recurrent wheezing group (t-test P=0.0024).
The off-line tidal breathing method is a feasible and appealing method for measuring exhaled nitric oxide in pre-school children. With this method, higher tidal exhaled nitric oxide levels were found in children with recurrent wheezing.
呼出一氧化氮（ENO）用作气道炎症的非侵入性标志物。本研究的目的是使用一种相对新颖的方法——离线潮气呼吸法，测量学龄前人群的ENO，并调查不同喘息表现的受试者之间ENO的差异。通过连接到呼气阻力为5 cm H₂O的双向阀的口罩，对129名儿童（中位年龄4.4岁，四分位数4.0 - 4.8岁）进行ENO测量。从三次重复测量中计算平均潮气ENO浓度（tENO）。89名对照受试者的平均±标准误tENO为13±0.4 ppb（95%置信区间11.8 - 13.7 ppb）；这一水平与15名有反复喘息病史的儿童的tENO有显著差异（18.6±1.9 ppb；95%置信区间14.5 - 22.7 ppb；t检验P<0.0001）。16名单次喘息发作儿童的平均tENO为11.4±1.0 ppb（95%置信区间9.2 - 13.6 ppb），因此与反复喘息组有显著差异（t检验P = 0.0024）。
离线潮气呼吸法是测量学龄前儿童呼出一氧化氮的一种可行且有吸引力的方法。使用这种方法，发现反复喘息儿童的潮气呼出一氧化氮水平更高。
