Vacca Giovanni, Colombo Giancarlo, Brunetti Giuliana, Melis Samuele, Molinari Daniela, Serra Salvatore, Seghizzi Roberto, Morazzoni Paolo, Bombardelli Ezio, Gessa Gian Luigi, Carai Mauro A M
Neuroscienze Scarl, Cagliari, Italy.
Phytother Res. 2003 May;17(5):537-41. doi: 10.1002/ptr.1203.
A previous study demonstrated that an extract of Salvia miltiorrhiza, a medicinal herb highly valued in Chinese folk medicine for the treatment of different pathologies, including insomnia, was capable of reducing voluntary alcohol intake in selectively bred Sardinian alcohol-preferring (sP) rats. The present study was designed to evaluate the suitability of different emulsifying, suspending agents and solvents as vehicles through which Salvia miltiorrhiza extracts can exert their reducing effect on alcohol intake. A single dose (100 mg/kg) of a standardised extract of Salvia miltiorrhiza was dissolved in either pure Polysorbate 80, arachis oil, PEG 400, or Polyoxyl 35 castor oil, or suspended in 0.5% CMC in water, and administered acutely by gavage to sP rats. A significant and specific reduction in alcohol intake was recorded only in rats treated with the combination of Polysorbate 80 plus the Salvia miltiorrhiza extract. A further experiment demonstrated that the ability of the combination of Polysorbate 80 in water plus the Salvia miltiorrhiza extract to decrease alcohol intake was dependent upon the concentration of Polysorbate 80. The results of the present study demonstrate that Polysorbate 80 is a proper vehicle for unravelling the reducing effect of Salvia miltiorrhiza extracts on alcohol intake. The ability of Polysorbate 80 to form micelles with the active ingredient(s) of the Salvia miltiorrhiza may explain these results. They may also offer relevant information for pharmaceutical preparation of Salvia miltiorrhiza extract to be used in future clinical trials.
先前的一项研究表明，丹参提取物能够降低选择性培育的撒丁岛嗜酒（sP）大鼠的自愿酒精摄入量。丹参是一种在中国民间医学中备受重视的草药，用于治疗包括失眠在内的多种病症。本研究旨在评估不同的乳化剂、悬浮剂和溶剂作为载体的适用性，通过这些载体，丹参提取物可以发挥其对酒精摄入量的降低作用。将单剂量（100 mg/kg）的标准化丹参提取物溶解于纯聚山梨醇酯80、花生油、聚乙二醇400或聚氧乙烯35蓖麻油中，或悬浮于0.5%的羧甲基纤维素钠水溶液中，然后通过灌胃急性给予sP大鼠。仅在接受聚山梨醇酯80加丹参提取物组合治疗的大鼠中记录到酒精摄入量显著且特异性降低。进一步的实验表明，聚山梨醇酯80水溶液加丹参提取物组合降低酒精摄入量的能力取决于聚山梨醇酯80的浓度。本研究结果表明，聚山梨醇酯80是揭示丹参提取物对酒精摄入量降低作用的合适载体。聚山梨醇酯80与丹参活性成分形成胶束的能力可能解释了这些结果。它们还可能为未来临床试验中使用的丹参提取物的药物制剂提供相关信息。
