Chowdhury R, Rashid R B, Sohrab M H, Hasan C M
Phytochemical Research Laboratory, Department of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh.
Pharmazie. 2003 Apr;58(4):272-3. doi: 10.1002/chin.200332126.
In a bioassay guided phytochemical investigation of Toona ciliata (Fam. Meliaceae), a new hydroxy steroidal ketone, 12alpha-hydroxystigmast-4-en-3-one (1) was isolated from the petroleum ether extract of the plant together with two known steroids and three C-methyl coumarins. The structure of 1 was established by means of spectroscopic analyses. Compound 1 was found cytotoxic in a brine shrimp lethality bioassay with LC50 of 9.9 microg/ml and it also showed significant antitumor activity with Ti50 value of 14.1 microg/ml in a potato disc bioassay.
