Arguelho Maria Lara P M, Andrade José F, Stradiotto Nelson R
Departamento de Química, Universidade Federal de Sergipe, 49100-000 São Cristóvão, SE, Brazil.
J Pharm Biomed Anal. 2003 Jun 1;32(2):269-75. doi: 10.1016/s0731-7085(02)00669-6.
Hydroxychloroquine (HCQ) is a halogenated aminoquinoline that presents wide biological activity, often being used as an antimalarial drug. The electrochemical reduction of HCQ was investigated by cyclic voltammetry and chronoamperometry using glassy carbon electrodes. By cyclic voltammetry, in acid medium, only the cathodic peak was observed. The electrochemical behavior of this peak is dependent on pH and the electrodic process occurs through an E(r)C(i) mechanism. The electron number (1e) consumed in the reduction of HCQ was obtained by chronoamperometry. A method for the electrochemical determination of HCQ in pharmaceutical tablets was developed using differential pulse voltammetry. The detection limit reached was 11.2 microg ml(-1) of HCQ with a relative standard deviation of 0.46%. A spectrophotometric study of HCQ has been also carried out utilizing a band at 343 nm. The obtained detection limit and the relative standard deviation were 0.1 microg ml(-1) and 0.36%, respectively. The electrochemical methods are sufficiently accurate and precise to be applied for HCQ determination, in laboratorial routine, which can be used to determine the drug at low level.
羟氯喹啉（HCQ）是一种具有广泛生物活性的卤代氨基喹啉，常被用作抗疟药物。采用玻碳电极，通过循环伏安法和计时电流法研究了HCQ的电化学还原。在循环伏安法中，在酸性介质中仅观察到阴极峰。该峰的电化学行为取决于pH值，且电极过程通过E(r)C(i)机制发生。通过计时电流法获得了HCQ还原过程中消耗的电子数（1e）。采用差分脉冲伏安法建立了一种测定药片中HCQ的电化学方法。达到的检测限为11.2 μg ml⁻¹的HCQ，相对标准偏差为0.46%。还利用343 nm处的吸收带对HCQ进行了分光光度研究。获得的检测限和相对标准偏差分别为0.1 μg ml⁻¹和0.36%。电化学方法足够准确和精密，可用于实验室常规测定HCQ，可用于低水平药物的测定。
