Khoobi Asma, Ghoreishi Sayed Mehdi, Behpour Mohsen, Shaterian Maryam, Salavati-Niasari Masoud
Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan P.O. Box 87317-51167, Islamic Republic of Iran.
Department of Analytical Chemistry, Faculty of Chemistry, University of Kashan, Kashan P.O. Box 87317-51167, Islamic Republic of Iran.
Colloids Surf B Biointerfaces. 2014 Nov 1;123:648-56. doi: 10.1016/j.colsurfb.2014.10.002. Epub 2014 Oct 16.
N,N'-bis[(E)-(1-pyridyl) methylidene]-1,3-propanediamine (PMPDA) self-assembled monolayer (SAM) was covalently prepared on a glassy carbon electrode (GCE). The electrode surface modification was characterized by scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Then GC-PMPDA SAM modified electrode was used to investigate the electrochemical behavior of hydroxychloroquine (HQ) using CV, double potential step chronocoulometry and linear sweep voltammetry (LSV) techniques. Using these techniques, the diffusion coefficient (D), electron transfer coefficient (α) and exchanging current density (j0) for HQ were calculated. Furthermore the modified electrode was applied as a high sensitive biosensor for determination of HQ in the presence of acetaminophen (AC). The GC-PMPDA SAM modified electrode provides two linear responses for HQ in the presence of AC in the concentration ranges from 0.09 to 10.21 μM and 10.21 to 98.29 μM by differential pulse voltammetry (DPV). The detection limit (three times the signal blank/slope) was 4.65 nM. Finally the modified electrode was satisfactorily used for determining of HQ in human body fluids.
在玻碳电极（GCE）上通过共价法制备了N,N'-双[(E)-(1-吡啶基)亚甲基]-1,3-丙二胺（PMPDA）自组装单层膜（SAM）。采用扫描电子显微镜（SEM）、电化学阻抗谱（EIS）和循环伏安法（CV）技术对电极表面修饰进行了表征。然后，使用CV、双电位阶跃计时库仑法和线性扫描伏安法（LSV）技术，利用GC-PMPDA SAM修饰电极研究了羟氯喹（HQ）的电化学行为。通过这些技术，计算了HQ的扩散系数（D）、电子转移系数（α）和交换电流密度（j0）。此外，该修饰电极还被用作高灵敏度生物传感器，用于在对乙酰氨基酚（AC）存在的情况下测定HQ。通过差分脉冲伏安法（DPV），GC-PMPDA SAM修饰电极在AC存在的情况下对HQ在0.09至10.21 μM和10.21至98.29 μM的浓度范围内提供了两个线性响应。检测限（三倍信号空白/斜率）为4.65 nM。最后，该修饰电极令人满意地用于测定人体体液中的HQ。
