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嗜盐菌紫膜蛋白发色团荧光的皮秒动力学

Picosecond kinetics of the fluorescence from the chromophore of the purple membrane protein of Halobacterium halobium.

作者信息

Alfano R R, Govindjee R, Becher B, Ebrey T G

出版信息

Biophys J. 1976 May;16(5):541-5. doi: 10.1016/S0006-3495(76)85709-8.

Abstract

The fluorescence emission kinetics at 740 nm of the retinylidence chromophore of the purple membrane protein of Halobacterium halobium have been studied. Using picosecond laser pulses and an optical Kerr gate, the fluorescence risetime is found to be less than 8 ps and its lifetime is 40 +/- 5 ps at 90 degrees K and is estimated to be less than 3 ps at room temperature.

摘要

对嗜盐菌紫膜蛋白视黄醛发色团在740纳米处的荧光发射动力学进行了研究。使用皮秒激光脉冲和光学克尔门,发现在90K时荧光上升时间小于8皮秒,其寿命为40±5皮秒,在室温下估计小于3皮秒。

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