细菌视紫红质的激发态动力学
Excited-state dynamics of bacteriorhodopsin.
作者信息
Kouyama T, Kinosita K, Ikegami A
出版信息
Biophys J. 1985 Jan;47(1):43-54. doi: 10.1016/S0006-3495(85)83875-3.
Abstract
Near infrared emission of bacteriorhodopsin at neutral pH and at room temperature was characterized by a large Stokes shift. This characteristic was lost in an acidic pH (approximately pH 2) where a remarkable enchancement (more than 10 times) in the fluorescence quantum yield accompanied the red shift in the main absorption band. It is suggested from fluorescence polarization measurements that the emission occurs from the first allowed excited state of the retinylidene chromophore, irrespective of pH. We suggest that the large Stokes shift observed at neutral pH is a result of a charge displacement (e.g., proton translocation) that occurs immediately after excitation, and is prevented by protonation (in the ground state) of an amino-acid residue in the protein.
摘要
在中性pH值和室温下，细菌视紫红质的近红外发射具有较大的斯托克斯位移。在酸性pH值（约pH 2）下，这种特性消失，此时荧光量子产率显著提高（超过10倍），同时主吸收带发生红移。荧光偏振测量表明，无论pH值如何，发射均来自视黄醛发色团的第一个允许激发态。我们认为，在中性pH值下观察到的大斯托克斯位移是激发后立即发生的电荷位移（例如质子转运）的结果，并且该电荷位移被蛋白质中氨基酸残基的质子化（基态）所阻止。
相似文献
1
Excited-state dynamics of bacteriorhodopsin.
Biophys J. 1985 Jan;47(1):43-54. doi: 10.1016/S0006-3495(85)83875-3.
2
[FLuorescence of photoactive bacteriorhodopsin].
Biofizika. 1981 Nov-Dec;26(6):964-72.
3
Photochemistry and fluorescence of bacteriorhodopsin excited in its 280-nm absorption band.
Biochemistry. 1981 Jan 6;20(1):205-9. doi: 10.1021/bi00504a034.
4
Fluorescence following excited-state protonation of riboflavin at N(5).
J Phys Chem B. 2013 May 9;117(18):5441-7. doi: 10.1021/jp312571d. Epub 2013 Apr 30.
5
Ultrafast proton shuttling in Psammocora cyan fluorescent protein.
J Phys Chem B. 2013 Sep 26;117(38):11134-43. doi: 10.1021/jp401114e. Epub 2013 Apr 19.
6
Photochemistry of a retinal protonated schiff-base analogue mimicking the opsin shift of bacteriorhodopsin.
J Phys Chem B. 2007 Mar 8;111(9):2327-34. doi: 10.1021/jp0669308. Epub 2007 Feb 14.
7
Picosecond and steady state, variable intensity and variable temperature emission spectroscopy of bacteriorhodopsin.
Biophys J. 1978 Sep;23(3):383-93. doi: 10.1016/S0006-3495(78)85457-5.
8
Chromophore equilibria in bacteriorhodopsin.
Biophys J. 1979 Nov;28(2):211-30. doi: 10.1016/S0006-3495(79)85172-3.
9
Photochemistry in dried polymer films incorporating the deionized blue membrane form of bacteriorhodopsin.
Biophys J. 1998 Oct;75(4):1619-34. doi: 10.1016/S0006-3495(98)77605-2.
10
The molecular mechanism of excitation in visual transduction and bacteriorhodopsin.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):549-53. doi: 10.1073/pnas.75.2.549.
引用本文的文献
1
Reversible Photochromic Reactions of Bacteriorhodopsin from at Femto- and Picosecond Times.
Molecules. 2024 Oct 13;29(20):4847. doi: 10.3390/molecules29204847.
2
Rational Design of Far-Red Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators: from Elucidation of the Fluorescence Mechanism in Archers to Novel Red-Shifted Variants.
ACS Phys Chem Au. 2024 Apr 29;4(4):347-362. doi: 10.1021/acsphyschemau.3c00073. eCollection 2024 Jul 24.
3
Fluorescence of the Retinal Chromophore in Microbial and Animal Rhodopsins.
Int J Mol Sci. 2023 Dec 8;24(24):17269. doi: 10.3390/ijms242417269.
4
Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway.
ACS Phys Chem Au. 2023 May 3;3(4):320-333. doi: 10.1021/acsphyschemau.3c00003. eCollection 2023 Jul 26.
5
Retinal chromophore charge delocalization and confinement explain the extreme photophysics of Neorhodopsin.
Nat Commun. 2022 Nov 4;13(1):6652. doi: 10.1038/s41467-022-33953-y.
6
Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming.
J Am Chem Soc. 2019 Jan 9;141(1):262-271. doi: 10.1021/jacs.8b09311. Epub 2018 Dec 28.
7
Fluorescence spectroscopy of rhodopsins: insights and approaches.
Biochim Biophys Acta. 2014 May;1837(5):694-709. doi: 10.1016/j.bbabio.2013.10.008. Epub 2013 Oct 29.
8
Aspartate-histidine interaction in the retinal schiff base counterion of the light-driven proton pump of Exiguobacterium sibiricum.
Biochemistry. 2012 Jul 24;51(29):5748-62. doi: 10.1021/bi300409m. Epub 2012 Jul 10.
9
Reconstitution of gloeobacter rhodopsin with echinenone: role of the 4-keto group.
Biochemistry. 2010 Nov 16;49(45):9792-9. doi: 10.1021/bi1014166. Epub 2010 Oct 26.
10
Reconstitution of Gloeobacter violaceus rhodopsin with a light-harvesting carotenoid antenna.
Biochemistry. 2009 Nov 24;48(46):10948-55. doi: 10.1021/bi901552x.
本文引用的文献
1
Location and orientation of the chromophore in bacteriorhodopsin. Analysis by fluorescence energy transfer.
J Mol Biol. 1981 Dec 5;153(2):337-59. doi: 10.1016/0022-2836(81)90282-5.
2
Dynamic structure of biological membranes as probed by 1,6-diphenyl-1,3,5-hexatriene: a nanosecond fluorescence depolarization study.
Biochemistry. 1981 Jul 21;20(15):4270-7. doi: 10.1021/bi00518a006.
3
Photophysics of light transduction in rhodopsin and bacteriorhodopsin.
Annu Rev Biophys Bioeng. 1981;10:315-54. doi: 10.1146/annurev.bb.10.060181.001531.
4
The analysis of fluorescence decay by a method of moments.
Biophys J. 1969 Nov;9(11):1337-50. doi: 10.1016/S0006-3495(69)86456-8.
5
Reversible dissociation of the purple complex in bacteriorhodopsin and identification of 13-cis and all-trans-retinal as its chromophores.
Eur J Biochem. 1973 Dec 17;40(2):453-63. doi: 10.1111/j.1432-1033.1973.tb03214.x.
6
Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.
Methods Enzymol. 1974;31:667-78. doi: 10.1016/0076-6879(74)31072-5.
7
Picosecond kinetics of the fluorescence from the chromophore of the purple membrane protein of Halobacterium halobium.
Biophys J. 1976 May;16(5):541-5. doi: 10.1016/S0006-3495(76)85709-8.
8
Primary photochemical processes in bacteriorhodopsin.
Biochem Biophys Res Commun. 1976 Feb 23;68(4):1109-15. doi: 10.1016/0006-291x(76)90310-7.
9
Observation of light emission from a rhodopsin.
Nature. 1976 Apr 22;260(5553):675-8. doi: 10.1038/260675a0.
10
Retinal has a highly dipolar vertically excited singlet state: implications for vision.
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2169-73. doi: 10.1073/pnas.73.7.2169.
Zotero 插件