细菌视紫红质的激发态动力学
Excited-state dynamics of bacteriorhodopsin.
作者信息
Kouyama T, Kinosita K, Ikegami A
出版信息
Biophys J. 1985 Jan;47(1):43-54. doi: 10.1016/S0006-3495(85)83875-3.
Abstract
Near infrared emission of bacteriorhodopsin at neutral pH and at room temperature was characterized by a large Stokes shift. This characteristic was lost in an acidic pH (approximately pH 2) where a remarkable enchancement (more than 10 times) in the fluorescence quantum yield accompanied the red shift in the main absorption band. It is suggested from fluorescence polarization measurements that the emission occurs from the first allowed excited state of the retinylidene chromophore, irrespective of pH. We suggest that the large Stokes shift observed at neutral pH is a result of a charge displacement (e.g., proton translocation) that occurs immediately after excitation, and is prevented by protonation (in the ground state) of an amino-acid residue in the protein.
摘要
在中性pH值和室温下，细菌视紫红质的近红外发射具有较大的斯托克斯位移。在酸性pH值（约pH 2）下，这种特性消失，此时荧光量子产率显著提高（超过10倍），同时主吸收带发生红移。荧光偏振测量表明，无论pH值如何，发射均来自视黄醛发色团的第一个允许激发态。我们认为，在中性pH值下观察到的大斯托克斯位移是激发后立即发生的电荷位移（例如质子转运）的结果，并且该电荷位移被蛋白质中氨基酸残基的质子化（基态）所阻止。
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本文引用的文献
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