一种测量光不稳定物种中皮秒现象的方法：细菌视紫红质的发射寿命。
A method for measuring picosecond phenomena in photolabile species: the emission lifetime of bacteriorhodopsin.
作者信息
Hirsch M D, Marcus M A, Lewis A, Mahr H, Frigo N
出版信息
Biophys J. 1976 Dec;16(12):1399-409. doi: 10.1016/S0006-3495(76)85783-9.
Abstract
We have measured the emission lifetime of bacteriorhodopsin at physiological temperatures to be 15 +/- 3 ps using a technique which employs a mode-locked dye laser, a sum frequency light gate, and a continuous flow system. We observe no concentration dependence of the lifetime over the range of 1.1 X 10(-4) M to 1.0 X 10(-5) M. We conclude that the emission which we observe comes from bacteriorhodopsin and not one of its photochemically produced intermediates, and that the emission cannot originate from the state into which light is absorbed.
摘要
我们使用一种技术，即采用锁模染料激光器、和频光闸以及连续流动系统，在生理温度下测量了细菌视紫红质的发射寿命为15±3皮秒。在1.1×10⁻⁴ M至1.0×10⁻⁵ M的浓度范围内，我们未观察到寿命与浓度有关。我们得出结论，我们观察到的发射来自细菌视紫红质，而非其光化学产生的中间体之一，并且该发射不可能源于吸收光的状态。
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本文引用的文献
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Reversible dissociation of the purple complex in bacteriorhodopsin and identification of 13-cis and all-trans-retinal as its chromophores.细菌视紫红质中紫色复合物的可逆解离以及13-顺式视黄醛和全反式视黄醛作为其发色团的鉴定。
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