Bell R G, Hazell L A, Sheridan J W
Cell Tissue Kinet. 1976 Jul;9(4):305-11. doi: 10.1111/j.1365-2184.1976.tb01278.x.
These experiments examined the effect of a diet limited only in protein (4% by weight) on haemopoietic stem cells in mice. This diet places severe restrictions on growth and cell proliferation and this was reflected in lower numbers of colony forming units (CFUs) and in vitro colony forming cells (CFCs). Differences were apparent in the response of different organs to this stress; for instance, the incidence of spleen CFUs fell sharply from around 40/mg spleen tissue to 1-4/mg spleen tissue after 3 weeks on a low protein diet. This selective loss did not occur in bone marrow where total CFUs remained proportional to cellular content. Yet a third pattern was shown by thymus CFUs--although the numbers were low these increased from 16/thymus in normal mice to 132/thymus in deprived mice. This was the only organ examined which showed an increase. The effects of a return to a high protein (18%) diet showed that the spleen was the most responsive organ. By day 5 after the return to 18% protein the spleen contained as many CFUs per million cells as the bone marrow. During this time the content of CFU in the spleen had increased some 50-fold whereas bone marrow CFUs only doubled. The spleen assumes the major reconstructive role during the refeeding process.
这些实验研究了仅蛋白质含量受限（占体重的4%）的饮食对小鼠造血干细胞的影响。这种饮食对生长和细胞增殖有严格限制，这反映在集落形成单位（CFU）和体外集落形成细胞（CFC）数量减少。不同器官对这种应激的反应存在明显差异；例如，低蛋白饮食3周后，脾脏CFU的发生率从约40个/毫克脾脏组织急剧下降至1 - 4个/毫克脾脏组织。这种选择性损失在骨髓中未出现，骨髓中的总CFU与细胞含量仍保持比例关系。胸腺CFU呈现出第三种模式——尽管数量较少，但从正常小鼠的16个/胸腺增加到缺乏营养小鼠的132个/胸腺。这是所检查的唯一显示增加的器官。恢复到高蛋白（18%）饮食的效果表明，脾脏是反应最灵敏的器官。恢复到18%蛋白质饮食后的第5天，脾脏每百万细胞中的CFU数量与骨髓相当。在此期间，脾脏中CFU的含量增加了约50倍，而骨髓CFU仅增加了一倍。在重新喂食过程中，脾脏承担了主要的重建作用。
