[商品茵陈中绿原酸的定性及定量方法]
[Qualitative and quantitative methods of chlorogenic acid in commercial Herba Artemisiae Scopariae].
作者信息
Song Xiao-jun, Zhang Yong-xin, Zhang Ying, Zhang Qi-wei
机构信息
First People' Hospital of Hangzhou, Hangzhou 310006, Zhejiang, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2002 Apr;27(4):267-9.
OBJECTIVE
To develop the identification and assay for chlorogenic acid in commercial Herba Artemisiae Scopariae.
METHOD
TLC method was used for identification with silica gel G plate and butyl acetate-formic acid-water (7:2.5:2.5) upper layer as a developing solvent. Chlorogenic acid in 85% methanolic extract was separated on the ODS column with methonal-3% acetic acid solution (15:85) as mobile phase. The detection wavelength was 327 nm.
RESULT
The qualitative method is repeatable. Chlorogenic acid in extracts is well separated, relationship of injection amount and peak area is linear (r = 0.9998) within the range of 0.075-0.6 microgram. The average recovery is 100.9% and repeatability is 1.4%. Ten samples purchased from different areas in the country were identified and quantified with the methods.
CONCLUSION
The methods and data could be used for quality control.
目的
建立商品茵陈中绿原酸的鉴别与含量测定方法。
方法
采用薄层色谱法鉴别，以硅胶G板，乙酸丁酯 - 甲酸 - 水（7:2.5:2.5）上层溶液为展开剂；采用高效液相色谱法测定，85%甲醇提取物中的绿原酸采用ODS柱分离，以甲醇 - 3%乙酸溶液（15:85）为流动相，检测波长为327nm。
结果
定性方法重复性良好。提取物中的绿原酸分离良好，在0.075 - 0.6μg范围内进样量与峰面积呈线性关系（r = 0.9998），平均回收率为100.9%，重复性为1.4%。采用所建立的方法对从全国不同地区购买的10个样品进行了鉴别和含量测定。
结论
所建立的方法及实验数据可用于茵陈的质量控制。
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