[枫香果实中桦木酸的定性与定量方法]
[Qualitative and quantitative methods of betulonic acid in fruits of Liquidambar formosana].
作者信息
Zhang Qi-Wei, Zhang Yong-Xin, Sun Yu-Ru, Zhang Dong
机构信息
Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 Aug;30(15):1168-70.
OBJECTIVE
To develop the identification and assay methods for betulonic acid in fruits of Liquidambar formosana.
METHOD
TLC method was used for identification with silica gel G plate and petroleum ether(60-90 degrees C)-acetone (17:3) as a developing solvent. Betulonic acid in ethanol extract was separated on the ODS column with methanol-water-glacial acetic acid (87:13:0.1) as mobile phase. Flow-rate was 1.0 mL x min(-1), and column temperature 35 degrees C. ELSD drift-tube temperature was 82 degrees C, and gas flow 1.25 L x min(-1).
RESULT
The qualitative method is repeatable. Betulonic acid in ethanol extracts is well separated, relationship of logarithms of injection amount and peak area is linear (r = 0.9997) within the range of 0.65-3.25 microg. The average recovery is 98.0% and RSD of repeatability is 2.5%. 11 crude drugs purchased from different areas in the country were identified and quantified with the methods.
CONCLUSION
The methods and data could be used for quality control of fruits of L. formosana.
目的
建立枫香果实中桦木酸的鉴别与含量测定方法。
方法
采用硅胶G板，以石油醚（60 - 90℃）-丙酮（17:3）为展开剂，用薄层色谱法进行鉴别。以甲醇-水-冰醋酸（87:13:0.1）为流动相，在ODS柱上分离乙醇提取物中的桦木酸。流速为1.0 mL·min⁻¹，柱温35℃。蒸发光散射检测器漂移管温度为82℃，气体流速为1.25 L·min⁻¹。
结果
定性方法具有重复性。乙醇提取物中的桦木酸分离良好，进样量对数与峰面积在0.65 - 3.25 μg范围内呈线性关系（r = 0.9997）。平均回收率为98.0%，重复性的相对标准偏差为2.5%。用该方法对从国内不同地区购买的11批药材进行了鉴别和定量。
结论
该方法及数据可用于枫香果实的质量控制。
Zotero 插件