流动相添加剂对反相高效液相色谱法分离四种生物活性化合物的影响。

Li Shengnan, Tian Minglei, Row Kyung Ho

Department of Chemical Engineering, Inha University, Incheon 402-751, Korea; E-Mails:

Int J Mol Sci. 2010 May 25;11(5):2229-40. doi: 10.3390/ijms11052229.

The use of mobile phase additives enhances the separation and resolution of the bioactive compounds on the C(18) column. Chlorogenic acid, caffeic acid, rutin, and scoparone from Herba Artemisiae Scopariae were investigated as the target compounds. Acetic acid, triethylamine, inorganic salts, and several ionic liquids were added as mobile phase additives into methanol/water (40:60, v/v). The result revealed that a mobile phase with 0.01 mol/L of ionic liquid [BMIM][BF(4)] enabled the optimum separation of the four target compounds.

流动相添加剂的使用提高了生物活性化合物在C(18)柱上的分离度和分辨率。研究了茵陈中的绿原酸、咖啡酸、芦丁和滨蒿内酯作为目标化合物。将乙酸、三乙胺、无机盐和几种离子液体作为流动相添加剂加入甲醇/水（40:60，v/v）中。结果表明，含有0.01 mol/L离子液体[BMIM][BF(4)]的流动相能够实现四种目标化合物的最佳分离。