在奥地利患者的乳腺癌组织中未检测到小鼠乳腺肿瘤病毒样env基因序列。

The mouse mammary tumor virus-like env gene sequence is not detectable in breast cancer tissue of Austrian patients.

作者信息

Witt Armin, Hartmann Beda, Marton Erika, Zeillinger Robert, Schreiber Martin, Kubista Ernst

机构信息

Department of Obstetrics and Gynecology, University of Vienna, Währinger Gürtel 18-20, A-1090 Vienna, Austria.

出版信息

Oncol Rep. 2003 Jul-Aug;10(4):1025-9.

PMID:12792764

Abstract

The mammary mouse tumor virus (MMTV) has been related to human breast cancer in previous studies, but these have yielded contradictory results. An MMTV env gene-like sequence was detectable in a relatively high proportion (38%) of human breast cancer tissues. The aim of this study was to determine the proportion of this 660 bp MMTV env gene-like sequence in a population of Austrian breast cancer patients. We performed PCR, repeat PCR, and nested PCR. We did not find any exogenous MMTV env gene sequences in the 50 DNA samples of human breast cancer tissue nor in 22 breast cancer cell lines including MCF-7, which has previously been described as a positive control.

摘要

在先前的研究中，乳腺小鼠肿瘤病毒（MMTV）已被认为与人类乳腺癌有关，但这些研究得出了相互矛盾的结果。在相对较高比例（38%）的人类乳腺癌组织中可检测到一种MMTV env基因样序列。本研究的目的是确定在奥地利乳腺癌患者群体中这种660 bp MMTV env基因样序列的比例。我们进行了PCR、重复PCR和巢式PCR。我们在50份人类乳腺癌组织DNA样本中以及在包括MCF-7在内的22种乳腺癌细胞系中均未发现任何外源性MMTV env基因序列，而MCF-7此前曾被描述为阳性对照。