墨西哥女性乳腺癌样本中的小鼠乳腺肿瘤病毒样基因序列。
Mouse mammary tumor virus-like gene sequences in breast cancer samples of Mexican women.
作者信息
Zapata-Benavides P, Saavedra-Alonso S, Zamora-Avila D, Vargas-Rodarte C, Barrera-Rodríguez R, Salinas-Silva J, Rodríguez-Padilla C, Tamez-Guerra R, Trejo-Avila L
机构信息
Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León (UANL), Ciudad Universitaria, San Nicolás de los Garza, NL, México.
出版信息
Intervirology. 2007;50(6):402-7. doi: 10.1159/000110652. Epub 2007 Oct 31.
BACKGROUND
Previous reports related the presence of mouse mammary tumor virus (MMTV)-like gene sequences to human breast carcinoma. The aim of this study was to determine whether MMTV-like env gene sequences are present in breast cancer samples of Mexican women and in breast and lung cancer cell lines.
METHODS
Using specific primers for MMTV, we tested 3 breast cancer cell lines, 4 non-small lung cancer cell lines and 119 breast cancer samples from Mexican women.
RESULTS
MMTV-like gene sequences were amplified in the lung cancer cell INER-51, but not in the MCF-7 cell line that has been used as a positive control in other reports and in 5 of 119 (4.2%) breast cancer biopsy tissues. Furthermore, the identity of sequences of PCR products from INER-51 and a breast cancer-positive sample are 98 and 99% when compared with the env region of MMTV (GenBank accession No. AY161347).
CONCLUSION
These results indicate that MMTV-like gene sequences are present in the Mexican population.
背景
先前的报告将小鼠乳腺肿瘤病毒（MMTV）样基因序列的存在与人类乳腺癌联系起来。本研究的目的是确定MMTV样env基因序列是否存在于墨西哥女性的乳腺癌样本以及乳腺癌和肺癌细胞系中。
方法
我们使用针对MMTV的特异性引物，检测了3种乳腺癌细胞系、4种非小细胞肺癌细胞系以及来自墨西哥女性的119份乳腺癌样本。
结果
在肺癌细胞系INER-51中扩增出了MMTV样基因序列，但在其他报告中用作阳性对照的MCF-7细胞系以及119份乳腺癌活检组织中的5份（4.2%）中未扩增出。此外，与MMTV的env区域（GenBank登录号AY161347）相比，INER-51和一份乳腺癌阳性样本的PCR产物序列同一性分别为98%和99%。
结论
这些结果表明MMTV样基因序列存在于墨西哥人群中。
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