人和大鼠肾脏中肝脏型和心脏型脂肪酸结合蛋白的分子鉴定。逆转录聚合酶链反应的应用。

Molecular identification of the liver- and the heart-type fatty acid-binding proteins in human and rat kidney. Use of the reverse transcriptase polymerase chain reaction.

作者信息

Maatman R G, van de Westerlo E M, van Kuppevelt T H, Veerkamp J H

机构信息

Department of Biochemistry, University of Nijmegen, The Netherlands.

出版信息

Biochem J. 1992 Nov 15;288 ( Pt 1)(Pt 1):285-90. doi: 10.1042/bj2880285.

The cDNAs of two types of fatty acid-binding protein (FABP) present in human kidney, previously described as types A and B, were isolated using reverse transcriptase-PCR (RT-PCR) with human kidney mRNA and various sets of primers. The cDNA fragments were cloned and sequenced. Renal FABP type A and B cDNAs appeared to be completely identical to human liver- and heart-type FABP cDNAs respectively. In the second part of this study we demonstrated the presence of liver-type FABP in rat kidney by chromatography, e.l.i.s.a. and immunocytochemistry. The ratio and cellular distribution of the two FABP types varies markedly in human and rat kidney. Using RT-PCR we were also able to prepare and identify liver- and heart-type FABP cDNAs with mRNA from both male and female rat kidney.

利用逆转录聚合酶链反应（RT-PCR），以人肾信使核糖核酸（mRNA）和多组引物分离出了先前被描述为A 型和B型的存在于人类肾脏中的两种脂肪酸结合蛋白（FABP）的互补脱氧核糖核酸（cDNA）。将这些cDNA片段进行克隆并测序。结果显示，肾脏A型FABP cDNA和B型FABP cDNA分别与人肝脏型和心脏型FABP cDNA完全相同。在本研究的第二部分，我们通过色谱法、酶联免疫吸附测定（ELISA）和免疫细胞化学方法证实了大鼠肾脏中存在肝脏型FABP。在人和大鼠肾脏中，这两种FABP类型的比例和细胞分布存在显著差异。利用RT-PCR，我们还能够从雄性和雌性大鼠肾脏的mRNA中制备并鉴定出肝脏型和心脏型FABP cDNA。