Wyczółkowska J, Sułowska Z, Dastych J
Department of Biogenic Amines of Polish Academy of Sciences, Lódź.
Exp Toxicol Pathol. 1992 Oct;44(6):349-53. doi: 10.1016/S0940-2993(11)80226-5.
The in vitro production of histamine releasing factor (HRF) by lymphoid cells of rats, both normal and infected with Nippostrongylus brasiliensis, has been studied. Spleen cells and thymocytes were cultured either alone or in the presence of mitogen (PHA, 10 and 50 micrograms/ml) and the dialysed cell-free supernatants were tested for histamine releasing activity on rat peritoneal and pleural mast cell in vitro. We found that spleen cells and thymocytes of normal rats stimulated with PHA in 24 h cultures generated a factor which released histamine and 5-hydroxytryptamine from mast cells, and this ability was potentiated following N. brasiliensis infection of rats - lymphoid cells donors. Pleural mast cells were more sensitive to the action of HRF than peritoneal cells. Rat HRF had an apparent m.w. of 50,000 to 70,000 daltons as determined by gel chromatography and was a heat stable protein inducing histamine release from homologous mast cells in a very rapid (complete in 1-2 min at 37 degrees C), dose and temperature dependent secretory process.
已经研究了正常大鼠以及感染巴西日圆线虫的大鼠的淋巴细胞在体外产生组胺释放因子（HRF）的情况。脾细胞和胸腺细胞单独培养或在有丝分裂原（PHA，10和50微克/毫升）存在的情况下培养，并且对透析后的无细胞上清液进行体外大鼠腹膜和胸膜肥大细胞组胺释放活性的检测。我们发现，在24小时培养中用PHA刺激的正常大鼠的脾细胞和胸腺细胞产生了一种能从肥大细胞释放组胺和5-羟色胺的因子，并且在大鼠（淋巴细胞供体）感染巴西日圆线虫后这种能力增强。胸膜肥大细胞比腹膜细胞对HRF的作用更敏感。通过凝胶色谱法测定，大鼠HRF的表观分子量为50,000至70,000道尔顿，是一种热稳定蛋白，在非常快速的（37℃下1 - 2分钟内完成）、剂量和温度依赖性分泌过程中诱导同源肥大细胞释放组胺。
