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莱氏无胆甾原体在核糖体RNA操纵子的16S-23S间隔区含有转运RNA基因。

Acholeplasma laidlawii has tRNA genes in the 16S-23S spacer of the rRNA operon.

作者信息

Nakagawa T, Uemori T, Asada K, Kato I, Harasawa R

机构信息

Biotechnology Research Laboratories, Takara Shuzo Co., Ltd., Shiga, Japan.

出版信息

J Bacteriol. 1992 Dec;174(24):8163-5. doi: 10.1128/jb.174.24.8163-8165.1992.

DOI:10.1128/jb.174.24.8163-8165.1992
PMID:1281149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC207559/
Abstract

We amplified the 16S-23S rRNA intergenic spacer region of Acholeplasma laidlawii PG8 by polymerase chain reaction (PCR) and obtained two specific PCR products in different sizes. We have sequenced both PCR products and found that one of them has sequence homologous to the spacer tRNA genes in Bacillus subtilis. This is the first evidence of tRNA genes between the 16S-23S rRNA intergenic spacer regions in members of the class Mollicutes.

摘要

我们通过聚合酶链反应（PCR）扩增了莱氏无胆甾原体PG8的16S - 23S rRNA基因间隔区，并获得了两种不同大小的特异性PCR产物。我们对这两种PCR产物都进行了测序，发现其中一种与枯草芽孢杆菌的间隔tRNA基因具有序列同源性。这是柔膜菌纲成员16S - 23S rRNA基因间隔区之间存在tRNA基因的首个证据。

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