Oguri Hiroki, Hirama Masahiro, Tsumuraya Takeshi, Fujii Ikuo, Maruyama Megumi, Uehara Hisatoshi, Nagumo Yoko
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan.
J Am Chem Soc. 2003 Jun 25;125(25):7608-12. doi: 10.1021/ja034990a.
Ciguatoxins are the major causative toxins of ciguatera seafood poisoning. Limited availability of ciguatoxins has hampered the development of a reliable and specific immunoassay for detecting these toxins in contaminated fish. Monoclonal antibodies (mAbs) specific against both ends of ciguatoxin CTX3C were prepared by immunization of mice with protein conjugates of rationally designed synthetic haptens, 3 and 4, in place of the natural toxin. Haptenic groups that possess a surface area larger than 400 A(2) were required to produce mAbs that can bind strongly to CTX3C itself. A direct sandwich enzyme-linked immunosorbent assay (ELISA) using these mAbs was established to detect CTX3C at the ppb level with no cross-reactivity against other related marine toxins, including brevetoxin A, brevetoxin B, okadaic acid, or maitotoxin.
雪卡毒素是雪卡鱼中毒的主要致病毒素。雪卡毒素的可得性有限，这阻碍了开发一种可靠且特异的免疫测定法来检测受污染鱼类中的这些毒素。通过用合理设计的合成半抗原3和4的蛋白质缀合物免疫小鼠，制备了针对雪卡毒素CTX3C两端的单克隆抗体（mAb），以替代天然毒素。需要具有大于400 Ų表面积的半抗原基团来产生能与CTX3C本身强烈结合的单克隆抗体。利用这些单克隆抗体建立了一种直接夹心酶联免疫吸附测定法（ELISA），以检测十亿分比水平的CTX3C，且对其他相关海洋毒素，包括短裸甲藻毒素A、短裸甲藻毒素B、冈田酸或刺尾鱼毒素无交叉反应。
