Department of Biological Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.
Toxicon. 2010 Oct;56(5):797-803. doi: 10.1016/j.toxicon.2009.06.003. Epub 2009 Jun 11.
Ciguatoxins are the major causative toxins of ciguatera seafood poisoning. Limited availability of ciguatoxins has hampered the development of a reliable and specific immunoassay for detecting these toxins in contaminated fish. Monoclonal antibodies (mAbs) specific against both ends of Pacific ciguatoxins CTX3C and 51-hydroxyCTX3C were prepared by immunization of mice with the protein conjugates of rationally designed synthetic haptens in place of the natural toxin. Haptenic groups that possess a surface area larger than 400 A(2) were required to produce mAbs that can bind strongly to CTX3C or 51-hydroxyCTX3C. A direct sandwich enzyme-linked immunosorbent assay (ELISA) using these mAbs was established to detect CTX3C and 51-hydroxyCTX3C at the ppb level with no cross-reactivity against the other marine toxins, including brevetoxin A, brevetoxin B, okadaic acid, or maitotoxin.
雪卡毒素是雪卡鱼中毒的主要致病毒素。由于雪卡毒素的供应有限，因此开发一种可靠且特异性的免疫检测方法来检测受污染鱼类中的这些毒素受到了阻碍。通过用合理设计的合成半抗原的蛋白质缀合物免疫小鼠，制备了针对太平洋雪卡毒素 CTX3C 和 51-羟基-CTX3C 两端的单克隆抗体（mAb）。需要具有大于 400 A（2）表面积的半抗原基团才能产生能够与 CTX3C 或 51-羟基-CTX3C 强烈结合的 mAb。使用这些 mAb 建立了直接夹心酶联免疫吸附测定（ELISA），可以在 ppb 水平检测 CTX3C 和 51-羟基-CTX3C，并且与其他海洋毒素（包括布雷毒素 A、布雷毒素 B、短裸甲藻毒素或鳗毒素）没有交叉反应。
