Cohen B B, Wallace M R, Crichton D N
MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.
Mol Cell Probes. 1992 Oct;6(5):439-42. doi: 10.1016/0890-8508(92)90039-z.
Dinucleotide repeat sequences ('microsatellites') have been used as polymorphic genetic markers following amplification in the polymerase chain reaction (PCR). We have compared several methods of analysing the PCR products. The most reliable and unambiguous results were obtained when the PCR products were probed with a specific dinucleotide repeat oligonucleotide, so that only the microsatellite-containing products were detectable.
二核苷酸重复序列（“微卫星”）在聚合酶链反应（PCR）中扩增后已被用作多态性遗传标记。我们比较了几种分析PCR产物的方法。当用特定的二核苷酸重复寡核苷酸探测PCR产物时，可获得最可靠且明确的结果，这样只有含微卫星的产物能够被检测到。
