Urbonaviciūte A, Jakstas V, Kornysova O, Janulis V, Maruska A
Department of Chemistry, Vytautas Magnus University, Vileikos st. 8, LT-44404 Kaunas, Lithuania.
J Chromatogr A. 2006 Apr 21;1112(1-2):339-44. doi: 10.1016/j.chroma.2006.01.034. Epub 2006 Jan 26.
Flavonoids are an important group of natural compounds, which can prevent coronary heart disease and have antioxidant properties. Hawthorn is a well known and widely used medicinal plant due to its cardiotonic activity. Previous studies refer mostly to the HPLC analysis of the flavonoids: vitexin, quercetin, hyperoside, oligomeric procyanidins, which appear to be primarily responsible for the cardiac action of the plant. Aqueous ethanolic extracts of single-styled hawthorn (Crataegus monogyna Jacq., f.: Rosaceae Juss.) leaves and sprouts were analyzed by means of capillary zone electrophoresis (CZE). Influence of vegetation period on the extract qualitative composition and flavonoids quantities was evaluated. Sample preparation by extraction using different concentration of aqueous ethanol (40-96%, v/v) and the influence of extractant composition on the recovery of flavonoids are discussed in detail. The results obtained using CZE are compared to the results of spectrophotometric and HPLC analysis of the extracts. The effect of storage conditions of extracts (solar irradiation, temperature and duration) on degradation of flavonoids was investigated.
黄酮类化合物是一类重要的天然化合物，具有预防冠心病和抗氧化的特性。山楂因其强心作用而成为一种广为人知且广泛使用的药用植物。以往的研究大多涉及黄酮类化合物的高效液相色谱分析：牡荆素、槲皮素、金丝桃苷、低聚原花青素，这些似乎是该植物心脏作用的主要成分。采用毛细管区带电泳（CZE）对单花山楂（山楂属单性花，蔷薇科）叶片和芽的乙醇水提取物进行了分析。评估了植被时期对提取物定性组成和黄酮类化合物含量的影响。详细讨论了使用不同浓度乙醇水（40 - 96%，v/v）进行提取的样品制备方法以及提取剂组成对黄酮类化合物回收率的影响。将毛细管区带电泳获得的结果与提取物的分光光度法和高效液相色谱分析结果进行了比较。研究了提取物储存条件（阳光照射、温度和持续时间）对黄酮类化合物降解的影响。
