Atmani F, Slimani Y, Mimouni M, Hacht B
Laboratory of Cellular Physiology and Pharmacology, Department of Biology, University Mohammed 1, Oujda, Morocco.
BJU Int. 2003 Jul;92(1):137-40. doi: 10.1046/j.1464-410x.2003.04289.x.
To evaluate the prophylactic potential of a herbal decoction from Herniaria hirsuta, a medicinal plant widely used in Morocco to treat kidney stones, by assessing the effect of oral administration in experimentally induced calcium oxalate (CaOx) nephrolithiasis in rats.
Two groups of six rats each were rendered nephrolithic by treating with ethylene glycol 0.75% and ammonium chloride 1% for 3 days, and then ethylene glycol only for 3 weeks. Maintained on ethylene glycol, one group of rats was also given 1 mL/day of the plant decoction, while the others received 1 mL of water instead for 2 weeks. Urine samples (24 h) were collected individually at 1, 3, 7, and 14 days for physicochemical analysis. On completing the treatment the kidneys were collected and analysed by light microscopy.
The water intake and diuresis decreased in the treated rats; there was no significant difference in urinary pH between the groups. Urinary chemistry was apparently unaffected by the plant extract, except for the magnesium content, which was higher in treated rats. Crystalluria was characterized by the excretion of large CaOx monohydrate and dihydrate crystals in untreated, but smaller crystals in treated rats. The histology showed large deposits of CaOx crystals in all parts of the kidney in untreated rats but with almost no deposits in those of treated rats.
H. hirsuta has an impressive prophylactic effect on CaOx stones in nephrolithic rats; the effect did not seem to be mediated by biochemical or diuretic changes.
通过评估口服给药对实验性诱导大鼠草酸钙（CaOx）肾结石的影响，来评价多毛墙草（Herniaria hirsuta）草药煎剂的预防潜力，多毛墙草是摩洛哥广泛用于治疗肾结石的一种药用植物。
两组大鼠，每组6只，通过给予0.75%乙二醇和1%氯化铵处理3天，然后仅给予乙二醇处理3周，诱导形成肾结石。在持续给予乙二醇的同时，一组大鼠每天还给予1 mL该植物煎剂，而另一组大鼠则给予1 mL水，持续2周。分别在第1、3、7和14天收集个体24小时尿液样本进行理化分析。治疗结束后，收集肾脏并通过光学显微镜进行分析。
处理后的大鼠水摄入量和尿量减少；两组之间尿液pH值无显著差异。除镁含量外，尿液化学成分显然不受植物提取物的影响，处理后的大鼠镁含量较高。结晶尿的特征是未处理大鼠排泄大量一水合和二水合CaOx晶体，而处理后的大鼠排泄的晶体较小。组织学显示，未处理大鼠肾脏各部位均有大量CaOx晶体沉积，而处理后的大鼠几乎没有沉积。
多毛墙草对肾结石大鼠的CaOx结石具有显著的预防作用；这种作用似乎不是由生化或利尿变化介导的。
