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Lam.叶提取物抗尿路结石作用的评估： 、 及方法。

Evaluation of Antiurolithiatic Effects of Lam. Leaves Extract: , and Approaches.

作者信息

Ali Hina, Jabeen Qaiser, Jamshed Ayesha, Ejaz Syeda Abida, Qadeer Maria, Anwaar Mariya, Rasheed Hafiz Muhammad Farhan

机构信息

Department of Pharmacology, Faculty of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, the Islamia University of Bahawalpur, Bahawalpur, Pakistan.

出版信息

Dose Response. 2024 Nov 12;22(4):15593258241301222. doi: 10.1177/15593258241301222. eCollection 2024 Oct-Dec.

DOI:10.1177/15593258241301222
PMID:39539938
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11558773/
Abstract

Lam. (Moringaceae), has traditionally been used for various renal diseases including urolithiasis. Considering the therapeutic and nutritional values, the present study was designed to investigate the antiurolithiatic potential of leaves through , and approaches. Methanolic aqueous extract of . leaves (MoL.Cr) was prepared and screened for phytoconstituents through FTIR and HPLC analysis, while antioxidant potential was determined by DPPH assay. Crystal nucleation, aggregation and growth assays were carried out to ascertain the inhibitory effects of MoL.Cr. Molecular docking was performed to analyze the interactions between phytoconstituents and targeted proteins (Glycolate oxidase, Albumin and Tamm-Horsfall). Whereas, ethylene glycol-induced urolithiasis model (1% ammonium chloride +0.75% ethylene glycol) was used for study. Presence of alkaloids, phenols, glycosides and flavonoids was confirmed by FTIR and HPLC analysis. MoL.Cr significantly inhibited the CaOx crystal nucleation, aggregation as well as growth and normalized urinary and serum parameters. Histological studies showed that MoL.Cr significantly restored hyperoxaluria-induced irregular epithelial lining, interstitial inflammation and dilated proximal tubules. Thus, demonstrated marked stone inhibiting potential which can be due to its antioxidant, lowering of urinary concentration of stone forming constituents and anti-crystallization effects.

摘要

辣木（辣木科）传统上被用于治疗包括尿路结石在内的各种肾脏疾病。考虑到其治疗和营养价值，本研究旨在通过体外、体内和分子对接方法研究辣木叶的抗尿路结石潜力。制备了辣木叶的甲醇水提取物（MoL.Cr），并通过傅里叶变换红外光谱（FTIR）和高效液相色谱（HPLC）分析筛选其植物成分，同时通过二苯基苦味酰基自由基（DPPH）测定法测定其抗氧化潜力。进行了晶体成核、聚集和生长试验，以确定MoL.Cr的抑制作用。进行分子对接以分析植物成分与靶向蛋白（乙醇酸氧化酶、白蛋白和Tamm-Horsfall蛋白）之间的相互作用。此外，使用乙二醇诱导的尿路结石模型（1%氯化铵+0.75%乙二醇）进行体内研究。FTIR和HPLC分析证实了生物碱、酚类、糖苷和黄酮类化合物的存在。MoL.Cr显著抑制草酸钙晶体的成核、聚集以及生长，并使尿液和血清参数恢复正常。组织学研究表明，MoL.Cr显著恢复了高草酸尿症引起的不规则上皮内衬、间质炎症和扩张的近端肾小管。因此，辣木叶显示出显著的结石抑制潜力，这可能归因于其抗氧化作用、降低结石形成成分的尿液浓度以及抗结晶作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/27184fecb22c/10.1177_15593258241301222-fig11.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/009edc16c156/10.1177_15593258241301222-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/dd5407de9783/10.1177_15593258241301222-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/2adf36c2c7de/10.1177_15593258241301222-fig8.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/d9b482c99d69/10.1177_15593258241301222-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/27184fecb22c/10.1177_15593258241301222-fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/da7fcda06947/10.1177_15593258241301222-img01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/08086a342d82/10.1177_15593258241301222-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/e96970e47be5/10.1177_15593258241301222-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/9648f8de9180/10.1177_15593258241301222-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/829c2b0a01ce/10.1177_15593258241301222-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/f2545b6a152b/10.1177_15593258241301222-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/009edc16c156/10.1177_15593258241301222-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/dd5407de9783/10.1177_15593258241301222-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/2adf36c2c7de/10.1177_15593258241301222-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/5f65ee23b24b/10.1177_15593258241301222-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/d9b482c99d69/10.1177_15593258241301222-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93a4/11558773/27184fecb22c/10.1177_15593258241301222-fig11.jpg

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