植物乳杆菌中分离出的两种隐蔽质粒的完整DNA序列及分析
Complete DNA sequence and analysis of two cryptic plasmids isolated from Lactobacillus plantarum.
作者信息
Daming Ren, Yinyu Wang, Zilai Wang, Jun Cui, Hekui Lan, Jingye Zhou
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, People's Republic of China.
出版信息
Plasmid. 2003 Jul;50(1):70-3. doi: 10.1016/s0147-619x(03)00010-6.
The complete nucleotide sequence of two cryptic plasmids isolated from Lactobacillus plantarum strain AS1.2986 has been determined. The smaller plasmid, designated pLP2000, encodes a 37.0kDa Rep protein and has a 17bp sequence repeated 10 times. Sequence analysis of the larger plasmid, designated pLP9000, revealed nine putative open reading frames (ORFs). Based on sequence similarity, ORF1 codes for a putative magnesium transporter protein that shows similarities to CorA from plasmid pCIS3 (Lactococcus lactis). None of the nine ORFs shows similarity to any known Rep protein. Southern blot analysis indicates these two plasmids both replicate via a rolling circle (RC) mechanism.
已测定从植物乳杆菌AS1.2986菌株分离出的两种隐蔽质粒的完整核苷酸序列。较小的质粒命名为pLP2000，编码一种37.0kDa的复制蛋白，有一段17bp的序列重复了10次。较大的质粒命名为pLP9000，序列分析显示有9个推定的开放阅读框（ORF）。基于序列相似性，ORF1编码一种推定的镁转运蛋白，与质粒pCIS3（乳酸乳球菌）的CorA相似。这9个ORF中没有一个与任何已知的复制蛋白相似。Southern杂交分析表明这两种质粒均通过滚环（RC）机制复制。
Zotero 插件